{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T01:22:57Z","timestamp":1747185777477,"version":"3.40.5"},"reference-count":36,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2020,5,27]],"date-time":"2020-05-27T00:00:00Z","timestamp":1590537600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100011002","name":"National Natural Science Foundation of China-Yunnan Joint Fund","doi-asserted-by":"publisher","award":["81700130"],"award-info":[{"award-number":["81700130"]}],"id":[{"id":"10.13039\/501100011002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>About 5 per cent of follicular lymphoma (FL) cases are double\u2010hit (DH) lymphomas. Double\u2010hit follicular lymphoma (DHFL) cell lines can improve our understanding and drug development on FL. But there are only few DHFL cell lines. Here, we established a new <jats:italic>MYC\/BCL2<\/jats:italic>\u00a0DHFL cell line, FL\u2010SJC. The cells were obtained from the hydrothorax of a patient with <jats:italic>MYC\/BCL2\u00a0<\/jats:italic>DHFL and cultured for 140 passages in vitro. FL\u2010SJC cells demonstrated CD19<jats:sup>++<\/jats:sup>, CD20<jats:sup>+<\/jats:sup>, CD22<jats:sup>++<\/jats:sup>, HLA\u2010DR<jats:sup>+<\/jats:sup>, CD10<jats:sup>+<\/jats:sup>, CD38<jats:sup>+<\/jats:sup>, Lambda<jats:sup>+<\/jats:sup> CD23<jats:sup>\u2010<\/jats:sup>, CD5<jats:sup>\u2010<\/jats:sup> and Kappa<jats:sup>\u2010<\/jats:sup>. The chromosome karyotypic analysis confirmed the co\u2010existence of t(8;22)(q24;q11) and t(14;18)(q32;q21), as well as additional abnormalities involving chromosomes 2 and 3. Fluorescence in situ hybridization analysis (FISH) showed <jats:italic>IGH\/BCL2<\/jats:italic> fusion gene and the <jats:italic>MYC<\/jats:italic> rearrangement. In addition, the FL\u2010SJC cells displayed <jats:italic>KMT2D\/MLL2<\/jats:italic> and <jats:italic>CREBBP<\/jats:italic> gene mutations. After subcutaneous inoculation of FL\u2010SJC cells, the SCID mice developed solid tumour masses within 6\u20108\u00a0weeks. FL\u2010SJC cells were proven to be free of Epstein\u2010Barr (EB) virus infection and be multidrug\u2010resistant. In a conclusion, the FL\u2010SJC cell line has been identified as a novel <jats:italic>MYC\/BCL2<\/jats:italic> double\u2010hit follicular lymphoma that can be used as a potentially available tool for the clinical and basic research, together with the drug development for <jats:italic>MYC\/BCL2<\/jats:italic> DHFL.<\/jats:p>","DOI":"10.1111\/jcmm.15425","type":"journal-article","created":{"date-parts":[[2020,5,27]],"date-time":"2020-05-27T12:55:39Z","timestamp":1590584139000},"page":"7928-7938","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Establishment and characterization of a novel \u2018double\u2010hit\u2019 follicular lymphoma cell line, FL\u2010SJC"],"prefix":"10.1111","volume":"24","author":[{"given":"Min","family":"Chen","sequence":"first","affiliation":[{"name":"Affiliated Hospital of Jiangsu University Zhenjiang 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