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The purpose of this study is to confirm the role of miR\u2010195 in arrhythmia caused by cardiac hypertrophy. The protein levels of Cav\u03b21, Kir2.1 and Kv4.3 in myocardium of HF mice were decreased. After miR\u2010195 was overexpressed in neonatal mice cardiomyocytes, the expression of ANP, BNP and \u03b2\u2010MHC was up\u2010regulated, and miR\u2010195 inhibitor reversed this phenomenon. Overexpression of miR\u2010195 reduced the estimated cardiac function of EF% and FS% in wild\u2010type (WT) mice. Transmission electron microscopy showed that the ultrastructure of cardiac tissues was damaged after miR\u2010195 overexpression by lentivirus in mice. miR\u2010195 overexpression increased the likelihood of arrhythmia induction and duration of arrhythmia in WT mice. Lenti\u2010miR\u2010195 inhibitor carried by lentivirus can reverse the decreased EF% and FS%, the increased incidence of arrhythmia and prolonged duration of arrhythmia induced by TAC in mice. After miR\u2010195 treatment, the protein expressions of Cav\u03b21, Kir2.1 and Kv4.3 were decreased in mice. The results were consistent at animal and cellular levels, respectively. Luciferase assay results showed that miR\u2010195 may directly target CACNB1, KCNJ2 and KCND3 to regulate the expression of Cav\u03b21, Kir2.1 and Kv4.3 proteins. MiR\u2010195 is involved in arrhythmia caused by cardiac hypertrophy by inhibiting Cav\u03b21, Kir2.1 and Kv4.3.<\/jats:p>","DOI":"10.1111\/jcmm.15431","type":"journal-article","created":{"date-parts":[[2020,5,29]],"date-time":"2020-05-29T00:09:35Z","timestamp":1590710975000},"page":"7991-8005","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Up\u2010regulation of miR\u2010195 contributes to cardiac hypertrophy\u2010induced arrhythmia by targeting calcium and potassium channels"],"prefix":"10.1111","volume":"24","author":[{"given":"Lina","family":"Xuan","sequence":"first","affiliation":[{"name":"Department of Pharmacology Harbin Medical University (the State\u2010Province Key Laboratories of Biomedicine\u2010Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education) College of Pharmacy Harbin Medical University Harbin, Heilongjiang 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