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Growth kinetics, in vitro competition and biofilm formation experiments demonstrated that SHV\u201012 ESBL contributed to a fitness benefit to <jats:italic>E cloacae<\/jats:italic> strain. The antibacterial activity of FSP (2.5, 5.0 and 10.0\u00a0\u03bcg\/mL) was tested against <jats:italic>E cloacae<\/jats:italic> bearing SHV\u201012 ESBL gene using bacterial sensitivity, agar bioassay and agar well diffusion assays. It was found that the addition of FSP demonstrated potent antibacterial activities against this bacterial as showed by the decrease of bacterial growth and the increase of the inhibition zone diameter. Furthermore, SHV\u201012 ESBL gene expression was decreased in <jats:italic>E cloacae<\/jats:italic> strain following different FSP treatment in a concentration\u2010dependent manner. In conclusion, these data showed that FSP exhibited potent good antibacterial activity against <jats:italic>E cloacae<\/jats:italic> producing SHV\u201012 ESBL via inhibition of SHV\u201012 ESBL gene expression, which may promote the development of novel natural antibacterial agents to treat infections caused by this drug\u2010resistant bacterial pathogen.<\/jats:p>","DOI":"10.1111\/jcmm.15510","type":"journal-article","created":{"date-parts":[[2020,6,26]],"date-time":"2020-06-26T03:18:55Z","timestamp":1593141535000},"page":"8763-8771","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Antibacterial potential of <i>Forsythia suspensa<\/i> polysaccharide against resistant <i>Enterobacter cloacae<\/i> with SHV\u201012 extended\u2010spectrum \u03b2\u2010lactamase (ESBL)"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2746-9303","authenticated-orcid":false,"given":"Jun","family":"Liu","sequence":"first","affiliation":[{"name":"Laboratory of Pathogenic Biology Guangdong Medical University Zhanjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liyao","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Surgery Affiliated Hospital of Guangdong Medical University Zhanjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhen","family":"Jia","sequence":"additional","affiliation":[{"name":"Department of Neurology Affiliated Hospital of Guangdong Medical University Zhanjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Chen","sequence":"additional","affiliation":[{"name":"Geriatrics Center Affiliated Hospital of Guangdong Medical University Zhanjiang 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