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The final effectors of this pathway are two transcriptional coactivators, yes\u2010associated protein (YAP) and transcriptional coactivator with PDZ\u2010binding motif (WWTR1 or TAZ). Previously, we showed aberrant activation of the Hippo pathway in autosomal\u2010dominant polycystic kidney disease (ADPKD), suggesting that YAP\/TAZ might play a role in disease progression. Using antisense oligonucleotides (ASOs) in a mouse model for ADPKD, we efficiently down\u2010regulated <jats:italic>Yap<\/jats:italic> levels in the kidneys. However, we did not see any effect on cyst formation or growth. Moreover, the expression of YAP\/TAZ downstream targets was not changed, while WNT and TGF\u2010\u03b2 pathways' downstream targets <jats:italic>Myc<\/jats:italic>, <jats:italic>Acta2<\/jats:italic> and <jats:italic>Vim<\/jats:italic> were more expressed after <jats:italic>Yap<\/jats:italic> knockdown. Overall, our data indicate that reducing YAP levels is not a viable strategy to modulate PKD progression.<\/jats:p>","DOI":"10.1111\/jcmm.15512","type":"journal-article","created":{"date-parts":[[2020,6,27]],"date-time":"2020-06-27T11:19:14Z","timestamp":1593256754000},"page":"8876-8882","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Reducing YAP expression in <i>Pkd1<\/i> mutant mice does not improve the cystic phenotype"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3263-3158","authenticated-orcid":false,"given":"Chiara","family":"Formica","sequence":"first","affiliation":[{"name":"Department of Human Genetics Leiden University Medical Center  Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Kunnen","sequence":"additional","affiliation":[{"name":"Department of Human Genetics Leiden University Medical Center  Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes G.","family":"Dauwerse","sequence":"additional","affiliation":[{"name":"Department of Human Genetics Leiden University Medical Center  Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam E.","family":"Mullick","sequence":"additional","affiliation":[{"name":"IONIS Pharmaceuticals  Carlsbad California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyra L.","family":"Dijkstra","sequence":"additional","affiliation":[{"name":"Department of Pathology Leiden University Medical Center  Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marion","family":"Scharpfenecker","sequence":"additional","affiliation":[{"name":"Department of Pathology Leiden University Medical Center  Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorien J. 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