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However, the specific mechanism is unknown. This study aimed to discover the related signal pathways of CARD9 in macrophages. SiRNA interference technology was used in vivo and in vitro to detect CARD9\u2010related signal pathways in peritoneal macrophages. Furthermore, Toll\u2010like receptor 4 (TLR4) and membrane\u2010associated C\u2010type lectin\u20101 (Dectin\u20101) pathways in macrophages were activated specially to looking for the upstream signal path of CARD9. Results showed up\u2010regulation of CARD9 expression in peritoneal macrophages of SAP rats (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05). CARD9 siRNA alleviated inflammatory cytokines, and inhibited the phosphorylation of NF\u2010\u03baB and p38MAPK in peritoneal macrophages in vivo or in vitro. Meanwhile, CARD9 siRNA reduced the concentration of CARD9 and Bcl10 in peritoneal macrophages, and TLR4 and Dectin\u20101 took part in CARD9 signal pathways in macrophages. In conclusion, there is an inflammation signal pathway comprised of TLR4\/Dectin\u20101\u2010CARD9\u2010NF\u2010\u03baB\/p38MAPK activated in macrophages in SAP. Blockade of CARD9 expression in macrophages can effectively alleviate SAP inflammation.<\/jats:p>","DOI":"10.1111\/jcmm.15559","type":"journal-article","created":{"date-parts":[[2020,8,13]],"date-time":"2020-08-13T06:22:28Z","timestamp":1597299748000},"page":"9774-9785","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Role of CARD9 in inflammatory signal pathway of peritoneal macrophages in severe acute pancreatitis"],"prefix":"10.1111","volume":"24","author":[{"given":"Jing","family":"Wang","sequence":"first","affiliation":[{"name":"Songjiang Hospital Affiliated to Shanghai Jiaotong University School of Medicine (Preparatory Stage) Shanghai China"},{"name":"Shanghai Songjiang Clinical Medical College of Nanjing Medical University Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Tian","sequence":"additional","affiliation":[{"name":"Songjiang Hospital Affiliated to Shanghai Jiaotong University School of Medicine (Preparatory Stage) Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang\u2010huan","family":"He","sequence":"additional","affiliation":[{"name":"Jinshan Hospital of Fudan University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7354-9617","authenticated-orcid":false,"given":"Zhi\u2010wen","family":"Yang","sequence":"additional","affiliation":[{"name":"Songjiang Hospital Affiliated to Shanghai Jiaotong University School of Medicine (Preparatory Stage) Shanghai China"},{"name":"Shanghai Songjiang Clinical Medical College of Nanjing Medical University Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin","family":"Wang","sequence":"additional","affiliation":[{"name":"Shanghai Songjiang Clinical Medical College of Nanjing Medical University Nanjing 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