{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:07:55Z","timestamp":1787015275606,"version":"build-2736575974"},"reference-count":34,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2020,7,8]],"date-time":"2020-07-08T00:00:00Z","timestamp":1594166400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Guangdong Provincial Academy of Traditional Chinese Medicine Research Project","award":["20173010"],"award-info":[{"award-number":["20173010"]}]},{"name":"Guangzhou Science and Technology Plan Project","award":["201802010030"],"award-info":[{"award-number":["201802010030"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    There is currently a lack of biomarkers to assist the diagnosis and prediction of primary gouty arthritis (PG). Therefore, we evaluated the clinical value of programmed cell death protein 1 (PD\u20101) mRNA expression in peripheral blood mononuclear cells (PBMCs) of patients with PG. This study included 36 patients with acute phase PG (APPG), 48 with non\u2010acute phase PG (NAPPG), 42 with asymptomatic hyperuricemia (AH) and 79 normal controls (NCs). PD\u20101 mRNA expression levels were detected by qRT\u2010PCR. PD\u20101 mRNA expression was statistically analysed by ANOVA or\n                    <jats:italic>t<\/jats:italic>\n                    tests, while correlations between PD\u20101 mRNA and clinical variables were assessed using Pearson correlation tests. Receiver operator characteristic (ROC) curve analysis was used to evaluate the diagnostic value of PD\u20101 in different PG stages. PD\u20101 mRNA expression was significantly lower in patients with APPG than that in NAPPG, AH and NCs (\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0&lt;\u00a00.01). Correlation analysis revealed that PD\u20101 mRNA levels correlated negatively with T\u2010score (\n                    <jats:italic>r<\/jats:italic>\n                    \u00a0=\u00a0\u22120.209,\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0&lt;\u00a00.01). ROC curve analysis showed that serum uric acid (SUA), PD\u20101 mRNA and both combined displayed higher diagnostic value in patients with PG, NAPPG and APPG compared to that in NCs and patients with non\u2010PG arthritis (NPG). Moreover, ROC curve analysis showed that SUA and PD\u20101 mRNA had good diagnostic value in APPG, with the greatest diagnostic power when combined. PD\u20101 mRNA could be a clinical auxiliary diagnostic biomarker for APPG, and the combined use of PD\u20101 mRNA and SUA is better than that of SUA alone.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.15582","type":"journal-article","created":{"date-parts":[[2020,7,8]],"date-time":"2020-07-08T07:46:42Z","timestamp":1594194402000},"page":"9323-9331","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["PD\u20101 mRNA expression in peripheral blood mononuclear cells as a biomarker for different stages of primary gouty arthritis"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7704-8593","authenticated-orcid":false,"given":"Jing","family":"Su","sequence":"first","affiliation":[{"name":"Third Affiliated Hospital of Guangzhou University of Chinese Medicine Guangzhou China"},{"name":"Guangzhou University of Chinese Medicine Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuefang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Hai Zhu Qu Jiang Hai Jie Community Health Service Center Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Zhao","sequence":"additional","affiliation":[{"name":"Third Affiliated Hospital of Guangzhou University of Chinese Medicine Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaodi","family":"Guo","sequence":"additional","affiliation":[{"name":"Third Affiliated Hospital of Guangzhou University of Chinese Medicine Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianxiong","family":"Wu","sequence":"additional","affiliation":[{"name":"Hai Zhu Qu Jiang Hai Jie Community Health Service Center Guangzhou 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