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This process is characterized by an increased expression of epithelial markers leading to a decreased invasion and metastatic rate. Based on the failure of conventional therapies, viral oncolysis might represent a promising alternative with canine distemper virus (CDV) as a possible candidate. This study hypothesizes that a CDV infection of canine histiocytic sarcoma cells (DH82 cells) triggers the MET process leading to a decreased cellular motility. Immunofluorescence and immunoblotting were used to investigate the expression of epithelial and mesenchymal markers followed by scratch assay and an invasion assay as functional confirmation. Furthermore, microarray data were analysed for genes associated with the MET process, invasion and angiogenesis. CDV\u2010infected cells exhibited an increased expression of epithelial markers such as E\u2010cadherin and cytokeratin 8 compared to controls, indicating a MET process. This was accompanied by a reduced cell motility and invasiveness. Summarized, these results suggest that CDV infection of DH82 cells triggers the MET process by an increased expression of epithelial markers resulting in a decreased cell motility in vitro.<\/jats:p>","DOI":"10.1111\/jcmm.15585","type":"journal-article","created":{"date-parts":[[2020,7,6]],"date-time":"2020-07-06T05:22:23Z","timestamp":1594012943000},"page":"9332-9348","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Mesenchymal to epithelial transition driven by canine distemper virus infection of canine histiocytic sarcoma cells contributes to a reduced cell motility in vitro"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2578-4409","authenticated-orcid":false,"given":"Federico","family":"Armando","sequence":"first","affiliation":[{"name":"Department of Pathology University of Veterinary Medicine Hannover Hannover Germany"},{"name":"Pathology Unit Department of Veterinary 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