{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T21:28:21Z","timestamp":1782422901838,"version":"3.54.5"},"reference-count":21,"publisher":"Wiley","issue":"19","license":[{"start":{"date-parts":[[2020,9,7]],"date-time":"2020-09-07T00:00:00Z","timestamp":1599436800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,9,7]],"date-time":"2020-09-07T00:00:00Z","timestamp":1599436800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Cytomegalovirus (CMV) is one of the most common intrauterine infection virus, which can cause intrauterine transmission through the placenta, resulting in abortion, stillbirth and congenital malformations. In this study, the co\u2010culture extravillous trophoblast (EVT) HTR8\/SVneo cell model of CMV infection was established in vitro. The toxicity of CMV infected EVT was determined, and then, the cell invasion experiment was conducted to evaluate the effect on the invasion ability of EVT cell lines. Western blot and real\u2010time PCR were used to detect the related cytokines in the PI3K\/AKT signalling pathway in cells. Flow cytometry was used to detect the immune function related factors of the supernatant of CMV culture on decidual NK cells. The TCID50 of CMV virus was 10\n                    <jats:sup>\u22125.4<\/jats:sup>\n                    . The results of immunofluorescence showed that a large number of fluorescent green of CMV pp65 antigen signals appeared in the cytoplasm of CMV infection group. CMV could infect and replicate EVT cells and inhibited cell proliferation. The expression of proteins PDK1, AKT\u2010S473 and AKT\u2010S308 was significantly increased in CMV infection group. The levels of IL\u201017, IL\u20104 and IFN\u2010\u03b3 were 8.7\u00a0\u00b1\u00a00.48%, 12.17\u00a0\u00b1\u00a00.61% and 6.66\u00a0\u00b1\u00a00.25%, respectively, in CMV infection group. The above results indicated that CMV infection inhibited EVT cells proliferation, weakened the invasion ability and inhibited the immune function of NK cells at the maternal\u2010fetal interface, resulting in the abnormal maternal\u2010fetal crosstalk.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.15638","type":"journal-article","created":{"date-parts":[[2020,10,21]],"date-time":"2020-10-21T04:30:43Z","timestamp":1603254643000},"page":"11170-11176","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Effects of cytomegalovirus infection on extravillous trophoblast cells invasion and immune function of NK cells at the maternal\u2010fetal interface"],"prefix":"10.1111","volume":"24","author":[{"given":"Xiaoqian","family":"Lin","sequence":"first","affiliation":[{"name":"Department of Obstetrics Fujian Maternity and Child Health Hospital Affiliated Hospital of Fujian Medical University Fuzhou China"},{"name":"Fujian Key Laboratory of Women and Children's Critical Diseases Research, Fujian Maternity and Child Health Hospital, Fujian Women and Children\u2019s Hospital Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusha","family":"Chen","sequence":"additional","affiliation":[{"name":"Cervical Disease Diagnosis and Treatment Health Center Fujian Maternity and Child Health Hospital Affiliated Hospital of Fujian Medical University Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuanji","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics Fujian Maternity and Child Health Hospital Affiliated Hospital of Fujian Medical University Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingshan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Obstetrics Fujian Maternity and Child Health Hospital Affiliated Hospital of Fujian Medical University Fuzhou 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