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Inflammation has been shown to be a critical factor in the progression of HF, regardless of the aetiology. Disappointingly, the majority of clinical trials targeting aspects of inflammation in patients with HF have been largely negative. Many clinical researches demonstrate that danshen has a good efficacy on HF, and however, whether danshen exerts anti\u2010inflammatory effects against HF remains unclear. In our study, the employment of a water extracted and alcohol precipitated of danshen extract attenuated cardiac dysfunction and inflammation response in acute myocardial infarction\u2010induced HF rats. Transcriptome technique and validation results revealed that TLR4 signalling pathway was involved in the anti\u2010inflammation effects of danshen. In vitro, danshen reduced the release of inflammatory mediators in LPS\u2010stimulated RAW264.7 macrophage cells. Besides, the LPS\u2010stimulated macrophage conditioned media was applied to induce cardiac H9C2 cells injury, which could be attenuated by danshen. Furtherly, knock\u2010down and overexpression of TLR4 were utilized to confirm that danshen ameliorated inflammatory injury via MyD88\u2010dependent TLR4\u2010TRAF6\u2010NF\u2010\u03baB signalling pathway in cardiomyocytes. Furthermore, by utilizing co\u2010immunoprecipitation, danshen was proved to suppress MD2\/TLR4 complex formation and MyD88 recruitment. In conclusion, our results demonstrated that danshen ameliorates inflammatory injury by controlling MD2\/TLR4\u2010MyD88 complex formation and TLR4\u2010TRAF6\u2010NF\u2010\u03baB signalling pathway in acute myocardial infarction\u2010induced HF.<\/jats:p>","DOI":"10.1111\/jcmm.15688","type":"journal-article","created":{"date-parts":[[2020,8,5]],"date-time":"2020-08-05T16:57:56Z","timestamp":1596646676000},"page":"10677-10692","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":62,"title":["Danshen (<i>Salvia miltiorrhiza<\/i>) restricts MD2\/TLR4\u2010MyD88 complex formation and signalling in acute myocardial infarction\u2010induced heart failure"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1370-490X","authenticated-orcid":false,"given":"Xiaoping","family":"Wang","sequence":"first","affiliation":[{"name":"School of Life Science Beijing University of Chinese Medicine Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongqing","family":"Guo","sequence":"additional","affiliation":[{"name":"School of Life Science Beijing University of Chinese Medicine Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weili","family":"Li","sequence":"additional","affiliation":[{"name":"School of Life Science Beijing University of Chinese Medicine Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Traditional Chinese Medicine Beijing University of Chinese Medicine Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanyan","family":"Jiang","sequence":"additional","affiliation":[{"name":"School of Life Science Beijing University of Chinese Medicine Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiyan","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Life Science Beijing University of Chinese Medicine Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun","family":"Li","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica Beijing University of Chinese Medicine Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Qiu","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Beijing Anzhen Hospital Capital Medical University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3652-8037","authenticated-orcid":false,"given":"Yong","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Life Science Beijing University of Chinese Medicine Beijing China"},{"name":"School of Traditional Chinese Medicine Beijing University of Chinese Medicine Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,8,5]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41569-019-0261-7"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchf.2017.09.015"},{"key":"e_1_2_10_4_1","first-page":"1841","article-title":"Pathophysiology of Myocardial Infarction. 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