{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T04:37:23Z","timestamp":1791607043136,"version":"4.3.4"},"reference-count":39,"publisher":"Wiley","issue":"18","license":[{"start":{"date-parts":[[2020,7,29]],"date-time":"2020-07-29T00:00:00Z","timestamp":1595980800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81701419"],"award-info":[{"award-number":["81701419"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81871145"],"award-info":[{"award-number":["81871145"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Endometriosis is a benign gynaecological disease appearing with pelvic pain, rising dysmenorrhoea and infertility seriously impacting on 10% of reproductive\u2010age females. This research attempts to demonstrate the function and molecular mechanism of RhoA\/ROCK pathway on epithelial\u2010mesenchymal transition (EMT) and proliferation in endometriosis. The expression of Rho family was abnormally changed in endometriotic lesions; in particular, RhoA and ROCK1\/2 were significantly elevated. Overexpression of RhoA in human eutopic endometrial epithelial cells (eutopic EECs) enhanced the cell mobility, epithelial\u2010mesenchymal transition (EMT) and proliferation, and RhoA knockdown exhibited the opposite function. Oestrogen up\u2010regulated the RhoA activity and expression of RhoA and ROCK1\/2. RhoA overexpression reinforced the effect of oestrogen on promoting EMT and proliferation, and RhoA knockdown impaired the effect of oestrogen. oestrogen receptor \u03b1 (ER\u03b1) was involved with the regulation of oestrogen on EMT and proliferation and up\u2010regulated RhoA activity and expression of RhoA and ROCK1\/2. The function of ER\u03b1 was modulated by the change in RhoA expression. Furthermore, phosphorylated ERK that was enhanced by oestrogen and ER\u03b1 promoted the protein expression of RhoA\/ROCK pathway. Endometriosis mouse model revealed that oestrogen enhanced the size and weight of endometriotic lesions. The expression of RhoA and phosphorylated ERK in mouse endometriotic lesions was significantly elevated by oestrogen. We conclude that abnormal activated RhoA\/ROCK pathway in endometriosis is responsible for the function of oestrogen\/ER\u03b1\/ERK signalling, which promoted EMT and proliferation and resulted in the development of endometriosis.<\/jats:p>","DOI":"10.1111\/jcmm.15689","type":"journal-article","created":{"date-parts":[[2020,7,29]],"date-time":"2020-07-29T13:38:04Z","timestamp":1596029884000},"page":"10693-10704","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["RhoA\/ROCK pathway mediates the effect of oestrogen on regulating epithelial\u2010mesenchymal transition and proliferation in endometriosis"],"prefix":"10.1111","volume":"24","author":[{"given":"Zhi\u2010Xiong","family":"Huang","sequence":"first","affiliation":[{"name":"School of Life Sciences Xiamen University Xiamen 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