{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,6]],"date-time":"2026-04-06T17:30:41Z","timestamp":1775496641432,"version":"3.50.1"},"reference-count":33,"publisher":"Wiley","issue":"18","license":[{"start":{"date-parts":[[2020,7,29]],"date-time":"2020-07-29T00:00:00Z","timestamp":1595980800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81702499, 81871869"],"award-info":[{"award-number":["81702499, 81871869"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2018M642326"],"award-info":[{"award-number":["2018M642326"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20170266"],"award-info":[{"award-number":["BK20170266"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012493","name":"Jiangsu Province Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2018K260C"],"award-info":[{"award-number":["2018K260C"]}],"id":[{"id":"10.13039\/100012493","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Conditionally replicative adenoviruses (CRAds) were promising approach for solid tumour treatment, but its oncolytic efficiency and toxicity are still not satisfactory for further clinical application. Here, we developed the CAIX promotor (CAIX<jats:sup>promotor<\/jats:sup>)\u2010controlled CRAd armed with a tumour suppressor absent in melanoma 2 (AIM2) to enhance its oncolytic potency. The CAIX<jats:sup>promotor<\/jats:sup>\u2010AIM2 adenoviruses (Ad\u2010CAIX<jats:sup>promotor<\/jats:sup>\u2010AIM2) could efficiently express E1A and AIM2 in renal cancer cells. Compared with Ad\u2010CAIX<jats:sup>promotor<\/jats:sup>, Ad\u2010CAIX<jats:sup>promotor<\/jats:sup>\u2010AIM2 significantly inhibited cell proliferation and enhanced cell apoptosis and cell killing, thus resulting in the oncolytic efficiency in 786\u2010O cells or OSRC\u20102 cells. To explore the therapeutic effect, various Ads were intratumourally injected into OSRC\u20102\u2010xenograft mice. The tumour growth was remarkably inhibited in Ad\u2010CAIX<jats:sup>promotor<\/jats:sup>\u2010AIM2\u2010treated group as demonstrated by reduced tumour volume and weight with a low toxicity. The inflammasome inhibitor YVAD\u2010CMK resulted in the reduction of anti\u2010tumour activity by Ad\u2010CAIX<jats:sup>promotor<\/jats:sup>\u2010AIM2 in vitro or in vivo, suggesting that inflammasome activation response was required for the enhanced therapeutic efficiency. Furthermore, lung metastasis of renal cancer mice was also suppressed by Ad\u2010CAIX<jats:sup>promotor<\/jats:sup>\u2010AIM2 treatment accompanied by the decreased tumour fossil in lung tissues. These results indicated that the tumour\u2010specific Ad\u2010CAIX<jats:sup>promotor<\/jats:sup>\u2010AIM2 could be applied for human renal cancer therapy. The therapeutic strategy of AIM2\u2010based CRAds could be a potential and promising approach for the therapy of primary solid or metastasis tumours.<\/jats:p>","DOI":"10.1111\/jcmm.15697","type":"journal-article","created":{"date-parts":[[2020,7,29]],"date-time":"2020-07-29T17:58:04Z","timestamp":1596045484000},"page":"10744-10755","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Absent in melanoma 2 enhances anti\u2010tumour effects of CAIX promotor controlled conditionally replicative adenovirus in renal cancer"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5155-5319","authenticated-orcid":false,"given":"Dafei","family":"Chai","sequence":"first","affiliation":[{"name":"Cancer Institute Xuzhou Medical University  Xuzhou China"},{"name":"Center of Clinical Oncology Affiliated Hospital of Xuzhou Medical University Xuzhou Medical University  Xuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong","family":"Qiu","sequence":"additional","affiliation":[{"name":"Department of Urology Affiliated Hospital of Xuzhou Medical University Xuzhou Medical University  Xuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zichun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Cancer Institute Xuzhou Medical University  Xuzhou China"},{"name":"Center of Clinical Oncology Affiliated Hospital of Xuzhou Medical University Xuzhou Medical University  Xuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shang","family":"Yuchen Shi","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology Affiliated Hospital of Xuzhou Medical University Xuzhou Medical University  Xuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gang","family":"Wang","sequence":"additional","affiliation":[{"name":"Cancer Institute Xuzhou Medical University  Xuzhou China"},{"name":"Center of Clinical Oncology Affiliated Hospital of Xuzhou Medical University Xuzhou Medical University  Xuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin","family":"Fang","sequence":"additional","affiliation":[{"name":"Cancer Institute Xuzhou Medical University  Xuzhou China"},{"name":"Center of Clinical Oncology Affiliated Hospital of Xuzhou Medical University Xuzhou Medical University  Xuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huizhong","family":"Li","sequence":"additional","affiliation":[{"name":"Cancer Institute Xuzhou Medical University  Xuzhou China"},{"name":"Center of Clinical Oncology Affiliated Hospital of Xuzhou Medical University Xuzhou Medical University  Xuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hailong","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Urology Affiliated Hospital of Xuzhou Medical University Xuzhou Medical University  Xuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Tian","sequence":"additional","affiliation":[{"name":"Cancer Institute Xuzhou Medical University  Xuzhou China"},{"name":"Center of Clinical Oncology Affiliated Hospital of Xuzhou Medical University Xuzhou Medical University  Xuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0208-6410","authenticated-orcid":false,"given":"Junnian","family":"Zheng","sequence":"additional","affiliation":[{"name":"Center of Clinical Oncology Affiliated Hospital of Xuzhou Medical University Xuzhou Medical University  Xuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,7,29]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2012.02380.x"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.1843"},{"key":"e_1_2_9_4_1","first-page":"642","article-title":"Oncolytic viruses: a new class of immunotherapy drugs","volume":"14","author":"Kaufman HL","year":"2015","journal-title":"Nat Rev"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2018.03.008"},{"key":"e_1_2_9_6_1","first-page":"689","article-title":"Optimizing oncolytic virotherapy in cancer treatment","volume":"18","author":"Harrington K","year":"2019","journal-title":"Nat Rev"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/14712598.2019.1595582"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/cas.13027"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40259-019-00367-0"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/15384047.2016.1190485"},{"key":"e_1_2_9_11_1","first-page":"3362","article-title":"A novel approach to glioma therapy using an oncolytic adenovirus with two specific promoters","volume":"15","author":"Liu F","year":"2018","journal-title":"Oncol Lett"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2010.03.015"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6690757"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11912-005-0036-7"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910380406"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/v2091844"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1209047"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.surg.2015.01.006"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature07725"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.06.001"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1859"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2018.07.021"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.13842"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cgt.7700739"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.28891"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3908"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/cgt.2010.52"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers10060202"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.omto.2018.10.005"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-09-3567"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/gt.2017.2"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1080\/2162402X.2015.1129484"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.00271"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.29040"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjcmm.15697","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.15697","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jcmm.15697","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.15697","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,20]],"date-time":"2023-08-20T17:00:01Z","timestamp":1692550801000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.15697"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,29]]},"references-count":33,"journal-issue":{"issue":"18","published-print":{"date-parts":[[2020,9]]}},"alternative-id":["10.1111\/jcmm.15697"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.15697","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,7,29]]},"assertion":[{"value":"2020-04-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-07-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-07-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}