{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:10:04Z","timestamp":1787015404912,"version":"build-2736575974"},"reference-count":38,"publisher":"Wiley","issue":"18","license":[{"start":{"date-parts":[[2020,8,16]],"date-time":"2020-08-16T00:00:00Z","timestamp":1597536000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81871762"],"award-info":[{"award-number":["81871762"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Brain\u2010derived neurotrophic factor (BDNF) has been reported to participate in fracture healing, whereas the mechanism is still unclear. Since osteoblast migration is important for fracture healing, investigating effects of BDNF on osteoblasts migration may help to reveal its mechanism. Here, MC3T3\u2010E1 cells were used in vitro while closed femur fracture mice were applied in vivo. Cells migration was assessed with Transwell assay. The protein expression was analysed by immunoblotting. X\u2010ray and Micro\u2010CT were performed at different time after fracture. Our results showed that BDNF promoted MC3T3\u2010E1 cells migration, integrin \u03b21 expression and ERK1\/2 and AKT phosphorylation. K252a, a specific inhibitor for TrkB, suppressed BDNF\u2010induced migration, integrin \u03b21 expression and activation of ERK1\/2 and AKT. PD98059 (an ERK1\/2 inhibitor) and LY294002 (an AKT inhibitor) both inhibited BDNF\u2010induced migration and integrin \u03b21 expression while integrin \u03b21 blocking antibody only suppressed cell migration. X\u2010ray and Micro\u2010CT analyses showed that the adenoviral carried integrin \u03b21 shRNA group had slower fracture healing at 7 and 21\u00a0days, but not 35\u00a0days compared to the control group. Thus, we proposed that BDNF stimulated MC3T3\u2010E1 cells migration by up\u2010regulating integrin \u03b21 via TrkB mediated ERK1\/2 and AKT signalling, and this may help to enhance the fracture healing.<\/jats:p>","DOI":"10.1111\/jcmm.15704","type":"journal-article","created":{"date-parts":[[2020,8,27]],"date-time":"2020-08-27T22:38:45Z","timestamp":1598567925000},"page":"10792-10802","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["BDNF promoted osteoblast migration and fracture healing by up\u2010regulating integrin \u03b21 via TrkB\u2010mediated ERK1\/2 and AKT signalling"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5862-7530","authenticated-orcid":false,"given":"Zitao","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Orthopedics The Affiliated Drum Tower Hospital of Nanjing University Medical School Nanjing 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