{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T19:58:18Z","timestamp":1758398298416,"version":"3.40.5"},"reference-count":25,"publisher":"Wiley","issue":"18","license":[{"start":{"date-parts":[[2020,8,14]],"date-time":"2020-08-14T00:00:00Z","timestamp":1597363200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004281","name":"Narodowe Centrum Nauki","doi-asserted-by":"publisher","award":["2017\/27\/B\/NZ5\/01540"],"award-info":[{"award-number":["2017\/27\/B\/NZ5\/01540"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>S\u00e9zary syndrome (SS) is an aggressive form of cutaneous T\u2010cell lymphoma (CTCL) characterized by the presence of circulating malignant CD4+\u00a0T cells (S\u00e9zary cells) with many complex changes in the genome, transcriptome and epigenome. Epigenetic dysregulation seems to have an important role in the development and progression of SS as it was shown that SS cells are characterized by widespread changes in DNA methylation. In this study, we show that the transmembrane protein coding gene <jats:italic>TMEM244<\/jats:italic> is ectopically expressed in all SS patients and SS\u2010derived cell lines and, to a lower extent, in mycosis fungoides and in a fraction of T\u2010cell lymphomas, but not in B\u2010cell malignancies and mononuclear cells of healthy individuals. We show that in patient samples and in the T\u2010cell lines <jats:italic>TMEM244<\/jats:italic> expression is negatively correlated with the methylation level of its promoter. Furthermore, we demonstrate that <jats:italic>TMEM244<\/jats:italic> expression can be activated in vitro by the CRISPR\u2010dCas9\u2013induced specific demethylation of <jats:italic>TMEM244<\/jats:italic> promoter region. Since both, <jats:italic>TMEM244<\/jats:italic> expression and its promoter demethylation, are not detected in normal lymphoid cells, they can be potentially used as markers in S\u00e9zary syndrome and some other T\u2010cell lymphomas.<\/jats:p>","DOI":"10.1111\/jcmm.15729","type":"journal-article","created":{"date-parts":[[2020,8,14]],"date-time":"2020-08-14T21:27:37Z","timestamp":1597440457000},"page":"10970-10977","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Hypomethylation of the promoter region drives ectopic expression of <i>TMEM244<\/i> in S\u00e9zary cells"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3855-4223","authenticated-orcid":false,"given":"Katarzyna","family":"I\u017cykowska","sequence":"first","affiliation":[{"name":"Institute of Human Genetics Polish Academy of Sciences  Pozna\u0144 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