{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T22:24:54Z","timestamp":1792621494814,"version":"4.5.0"},"reference-count":57,"publisher":"Wiley","issue":"19","license":[{"start":{"date-parts":[[2020,9,11]],"date-time":"2020-09-11T00:00:00Z","timestamp":1599782400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,9,11]],"date-time":"2020-09-11T00:00:00Z","timestamp":1599782400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The abundance of inflammatory mediators in injured joint indicates innate immune reactions activated during temporomandibular joint osteoarthritis (TMJOA) progression. Toll\u2010like receptor 4 (TLR4) can mediate innate immune reaction. Herein, we aimed to investigate the expression profile and effect of TLR4 in the cartilage and subchondral bone of the discectomy\u2010induced TMJOA mice. The expression of TLR4 and NF\u03baB p65 in the synovium of TMJOA patients was measured by immunohistochemistry, Western blotting and RT\u2010PCR. H&amp;E and Masson staining were utilized to assess the damage of cartilage and subchondral bone of the discectomy\u2010induced TMJOA mice. A TLR4 inhibitor, TAK\u2010242, was used to assess the effect of TLR4 in the cartilage and subchondral bone of the discectomy\u2010induced TMJOA mice by Safranin O, micro\u2010CT, immunofluorescence and immunohistochemistry. Western blotting was used to quantify the expression and effect of TLR4 in IL\u20101\u03b2\u2013induced chondrocytes. The expression of TLR4 and NF\u03baB p65 was elevated in the synovium of TMJOA patients, compared with the normal synovium. TLR4 elevated in the damaged cartilage and subchondral bone of discectomy\u2010induced TMJOA mice, and the rate of TLR4 expressing chondrocytes positively correlated with OA score. Intraperitoneal injections of TAK\u2010242 ameliorate the extent of TMJOA. Furthermore, TLR4 promotes the expression of MyD88\/NF\u03baB, pro\u2010inflammatory and catabolic mediators in cartilage of discectomy\u2010induced TMJOA. Besides, TLR4 participates in the production of MyD88\/NF\u03baB, pro\u2010inflammatory and catabolic mediators in IL\u20101\u03b2\u2013induced chondrocytes. TLR4 contributes to the damage of cartilage and subchondral bone in discectomy\u2010induced TMJOA mice through activation of MyD88\/NF\u03baB and release of pro\u2010inflammatory and catabolic mediators.<\/jats:p>","DOI":"10.1111\/jcmm.15763","type":"journal-article","created":{"date-parts":[[2020,9,25]],"date-time":"2020-09-25T11:24:26Z","timestamp":1601033066000},"page":"11489-11499","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["TLR4 contributes to the damage of cartilage and subchondral bone in discectomy\u2010induced TMJOA mice"],"prefix":"10.1111","volume":"24","author":[{"given":"Xin","family":"Liu","sequence":"first","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education School &amp; Hospital of Stomatology Wuhan University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heng\u2010xing","family":"Cai","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education School &amp; Hospital of Stomatology Wuhan University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pin\u2010yin","family":"Cao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases &amp; National Clinical Research Center for Oral Diseases &amp; Department of Orthognathic and TMJ Surgery West China Hospital of Stomatology Sichuan University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaping","family":"Feng","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education School &amp; Hospital of Stomatology Wuhan University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heng\u2010hua","family":"Jiang","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education School &amp; Hospital of Stomatology Wuhan University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Liu","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education School &amp; Hospital of Stomatology Wuhan University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Ke","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education School &amp; Hospital of Stomatology Wuhan University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5794-1117","authenticated-orcid":false,"given":"Xing","family":"Long","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education School &amp; Hospital of Stomatology Wuhan University Wuhan China"},{"name":"Department of Oral and Maxillofacial Surgery School and Hospital of Stomatology Wuhan University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,9,11]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"CD007261","article-title":"Interventions for the management of temporomandibular joint osteoarthritis","volume":"4","author":"De Souza RF","year":"2012","journal-title":"Cochrane Database Syst Rev"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/154405910808700406"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13191-013-0321-3"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2842.2009.02026.x"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2016.06.011"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034514560588"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2014.209"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar4449"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.11-184861"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00480.2015"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0142921"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.21951"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.20678"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13075-015-0833-9"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034515574770"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/978463"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1177\/154411130201300204"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1177\/1040638717728545"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11914-018-0447-7"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/7694921"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.12659\/MSM.908526"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345920710060501"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1834-7819.1997.tb00108.x"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcms.2017.05.029"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joms.2017.12.019"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0278-2391(91)90407-D"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2018.06.002"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2009.00297.x"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.110.068064"},{"key":"e_1_2_9_31_1","first-page":"556","article-title":"A time\u2010dependent degeneration manner of condyle in rat CFA\u2010induced inflamed TMJ","volume":"8","author":"Xu L","year":"2016","journal-title":"Am J Transl Res"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2009.01.002"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2020.173189"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2018.12.007"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2018.09.010"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/329405"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcms.2018.04.026"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature09121"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a006049"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00005-013-0243-0"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/ejp.1143"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2018.04.065"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2015.07.022"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1177\/154405910708600412"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.3899\/jrheum.140382"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2008.04.004"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1111\/jop.12401"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/cpr.12692"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2016.04.005"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10439-009-9696-z"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2017.08.009"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1177\/1947603520934858"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2018.01378"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2011.12.006"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.07.014"},{"key":"e_1_2_9_56_1","article-title":"Dioscin alleviates alcoholic liver fibrosis by attenuating hepatic stellate cell activation via the TLR4\/MyD88\/NF\u2010kappaB signaling pathway","volume":"5","author":"Liu M","year":"2015","journal-title":"Sci Rep"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-2094-11-59"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/2641248"}],"updated-by":[{"DOI":"10.1111\/jcmm.71263","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000}}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.15763","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jcmm.15763","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.15763","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T10:09:17Z","timestamp":1771409357000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.15763"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9,11]]},"references-count":57,"journal-issue":{"issue":"19","published-print":{"date-parts":[[2020,10]]}},"alternative-id":["10.1111\/jcmm.15763"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.15763","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,9,11]]},"assertion":[{"value":"2020-02-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-08-05","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-09-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}