{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T18:18:26Z","timestamp":1782584306582,"version":"3.54.5"},"reference-count":43,"publisher":"Wiley","issue":"19","license":[{"start":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T00:00:00Z","timestamp":1598918400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T00:00:00Z","timestamp":1598918400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Extracellular vesicle (EV)\u2010associated microRNAs (miRNAs) have been found as the important biomarkers participating in the development of osteonecrosis of the femoral head (ONFH). Consequently, this study sought to examine the underlying mechanism of bone marrow mesenchymal stem cell (BMSC)\u2010derived EVs containing miR\u2010148a\u20103p in ONFH. The ONFH rat models were established. Reverse transcription quantitative polymerase chain reaction (RT\u2010qPCR) and Western blot analysis were applied to detect miR\u2010148a\u20103p, Smad ubiquitination regulatory factor 1 (SMURF1), SMAD7 and B\u2010cell CLL\/lymphoma 2 (BCL2) expression, followed by determination of relationship between miR\u2010148a\u20103p and SMURF1. BMSCs were isolated from normal rats and ONFH rats, and EVs were extracted from BMSCs of normal rats. BMSCs from ONFH rats were treated with mimic, inhibitor, small interfering RNA or EVs from miR\u2010148a\u20103p mimic\u2010treated BMSCs from normal rats (BMSC\u2010EV\u2010miR\u2010148a\u20103p mimic). Cell Counting Kit\u20108 and alizarin red staining were utilized to detect cell viability and osteogenic differentiation of BMSCs. ONFH rats were injected with BMSC\u2010EV\u2010miR\u2010148a\u20103p mimic to explore the function of BMSC\u2010EV\u2010delivered miR\u2010148a\u20103p in vivo. miR\u2010148a\u20103p was down\u2010regulated in BMSCs and EVs from ONFH rats following decreased BMSCs viability and osteogenic differentiation. SMURF1 was a target gene of miR\u2010148a\u20103p, and resulted in ubiquitination and degradation of SMAD7 to decreased BCL2 expression. The proliferation and differentiation of BMSCs were promoted by BMSC\u2010EV\u2010miR\u2010148a\u20103p mimic or SMURF1 silencing. Additionally, BMSC\u2010EV\u2010miR\u2010148a\u20103p mimic increased cell proliferation and osteogenic response, diminished SMURF1 expression, and elevated SMAD7 and BCL2 expression in ONFH rats. Collectively, miR\u2010148a\u20103p overexpressed in BMSC\u2010EVs promoted SMAD7 and BCL2 expression by inhibiting SMURF1, thus alleviating ONFH.<\/jats:p>","DOI":"10.1111\/jcmm.15766","type":"journal-article","created":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T16:33:23Z","timestamp":1598978003000},"page":"11512-11523","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["microRNA\u2010148a\u20103p in extracellular vesicles derived from bone marrow mesenchymal stem cells suppresses SMURF1 to prevent osteonecrosis of femoral head"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8327-1660","authenticated-orcid":false,"given":"Shengxiang","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Orthopedics Hunan Children's Hospital Changsha 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