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A rat model of CAVD was established by administration of warfarin plus vitamin K. The expression levels of miR\u201029b, osteogenic markers and other genes were determined by qRT\u2010PCR, Western blot and\/or immunofluorescence and immunohistochemistry. The calcium content and alkaline phosphatase (ALP) activity were measured. The calcium content, ALP activity and osteogenic markers levels in calcified aorta and aortic valve were augmented compared to controls. The expression of miR\u201029b, p\u2010Smad3, and Wnt3 and \u03b2\u2010catenin was significantly up\u2010regulated, whereas TGF\u2010\u03b23 was markedly down\u2010regulated. However, compared with the CAVD model group, the calcium content and ALP activity in rats treated with antagomiR\u201029b were significantly decreased, and antagomiR\u201029b administration reversed the effects of CAVD model on the expression of miR\u201029b and osteogenic markers. Inhibition of miR\u201029b in CAVD rats prevented from vascular and valvular calcification and induced TGF\u2010\u03b23 expression, suggesting that the miR\u201029b\/TGF\u2010\u03b23 axis may play a regulatory role in the pathogenesis of vascular and valvular calcification and could play a significant role in the treatment of CAVD and other cardiovascular diseases.<\/jats:p>","DOI":"10.1111\/jcmm.15770","type":"journal-article","created":{"date-parts":[[2020,9,2]],"date-time":"2020-09-02T15:31:35Z","timestamp":1599060695000},"page":"11546-11557","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["AntagomiR\u201029b inhibits vascular and valvular calcification and improves heart function in rats"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4103-2063","authenticated-orcid":false,"given":"Ming","family":"Fang","sequence":"first","affiliation":[{"name":"Department of Cardiology 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