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However, the exact function and molecular mechanisms of NF\u2010YA in the progression of cervical cancer remains poorly understood. Here, the expression of NF\u2010YA detected by immunohistochemistry was gradually increased from normal cervical tissues, to the high\u2010grade squamous intraepithelial lesions, and then to cervical cancer tissues. NF\u2010YA promoted the cell proliferation and tumorigenic properties of cervical cancer cells as well as tumorsphere formation and chemoresistance <jats:italic>in vitro<\/jats:italic>. The luciferase reporter assay combined with mutagenesis analyses and Western blotting showed that NF\u2010YA trans\u2010activated the expression of SOX2 in cervical cancer. Furthermore, quantitative chromatin immunoprecipitation (qChIP) and electrophoretic mobility shift assay (EMSA) confirmed that NF\u2010YA protein directly bound to the CCAAT box region located upstream of the SOX2 promoter. Together, our data demonstrated that NF\u2010YA was highly expressed in cervical cancer and promoted the cell proliferation, tumorigenicity and CSC characteristic by trans\u2010activating the expression of SOX2.<\/jats:p>","DOI":"10.1111\/jcmm.15777","type":"journal-article","created":{"date-parts":[[2020,9,21]],"date-time":"2020-09-21T00:59:41Z","timestamp":1600649981000},"page":"12464-12475","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["NF\u2010YA promotes the cell proliferation and tumorigenic properties by transcriptional activation of SOX2 in cervical cancer"],"prefix":"10.1111","volume":"24","author":[{"given":"Wen\u2010Ting","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Reproductive Medicine The First Affiliated Hospital of Xi\u2019an Jiaotong University Shaanxi, Xi\u2019an 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