{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T02:43:31Z","timestamp":1789872211526,"version":"4.0.1"},"reference-count":47,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T00:00:00Z","timestamp":1598918400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81903293"],"award-info":[{"award-number":["81903293"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["LQ19H040003"],"award-info":[{"award-number":["LQ19H040003"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The pathophysiology of polycystic ovary syndrome (PCOS) is characterized by granulosa cell (GC) dysfunction. m\n                    <jats:sup>6<\/jats:sup>\n                    A modification affects GC function in patients with premature ovarian insufficiency (POI), but the role of m\n                    <jats:sup>6<\/jats:sup>\n                    A modification in PCOS is unknown. The purpose of the prospective comparative study was to analyse the m\n                    <jats:sup>6<\/jats:sup>\n                    A profile of the luteinized GCs from normovulatory women and non\u2010obese PCOS patients following controlled ovarian hyperstimulation. RNA m\n                    <jats:sup>6<\/jats:sup>\n                    A methylation levels were measured by m\n                    <jats:sup>6<\/jats:sup>\n                    A quantification assay in the luteinized GCs of the controls and PCOS patients. Then, m\n                    <jats:sup>6<\/jats:sup>\n                    A profiles were analysed by methylated RNA immunoprecipitation sequencing (MeRIP\u2010seq). We reported that the m\n                    <jats:sup>6<\/jats:sup>\n                    A level was increased in the luteinized GCs of PCOS patients. Comparative analysis revealed differences between the m\n                    <jats:sup>6<\/jats:sup>\n                    A profiles from the luteinized GC of the controls and PCOS patients. We identified\n                    <jats:italic>FOXO3<\/jats:italic>\n                    mRNA with reduced m\n                    <jats:sup>6<\/jats:sup>\n                    A modification in the luteinized GCs of PCOS patients. Selectively knocking down m\n                    <jats:sup>6<\/jats:sup>\n                    A methyltransferases or demethylases altered expression of FOXO3 in the luteinized GCs from the controls, but did not in PCOS patients. These suggested an absence of m\n                    <jats:sup>6<\/jats:sup>\n                    A\u2010mediated transcription of\n                    <jats:italic>FOXO3<\/jats:italic>\n                    in the luteinized GCs of PCOS patients. Furthermore, we demonstrated that the involvement of m\n                    <jats:sup>6<\/jats:sup>\n                    A in the stability of the\n                    <jats:italic>FOXO3<\/jats:italic>\n                    mRNA that is regulated via a putative methylation site in the 3\u2019\u2010UTR only in the luteinized GCs of the controls. In summary, our findings showed that altered m\n                    <jats:sup>6<\/jats:sup>\n                    A modification was involved in up\u2010regulated expression of\n                    <jats:italic>FOXO3<\/jats:italic>\n                    mRNA in the luteinized GCs from non\u2010obese PCOS patients following controlled ovarian hyperstimulation.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.15807","type":"journal-article","created":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T10:07:12Z","timestamp":1598954832000},"page":"11874-11882","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":50,"title":["Altered m\n                    <sup>6<\/sup>\n                    A modification is involved in up\u2010regulated expression of\n                    <i>FOXO3<\/i>\n                    in luteinized granulosa cells of non\u2010obese polycystic ovary syndrome 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