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By using proteomics approaches, we found that UDP\u2010glucose dehydrogenase (UGDH) was up\u2010regulated in highly metastatic ovarian cancer TOV21G cells, characterized by high invasiveness (TOV21G<jats:sup>HI<\/jats:sup>), in comparison to its parental control. Previous reports demonstrated that UGDH is involved in cell migration, but its specific role in cancer metastasis remains unclear. By performing immunohistochemical staining with tissue microarray, we found overexpression of UGDH in ovarian cancer tissue, but not in normal adjacent tissue. Silencing using RNA interference (RNAi) was utilized to knockdown UGDH, which resulted in a significant decrease in metastatic ability in transwell migration, transwell invasion and wound healing assays. The knockdown of UGDH caused cell cycle arrest in the G<jats:sub>0<\/jats:sub>\/G<jats:sub>1<\/jats:sub> phase and induced a massive decrease of tumour formation rate in vivo. Our data showed that UGDH\u2010depletion led to the down\u2010regulation of epithelial\u2010mesenchymal transition (EMT)\u2010related markers as well as MMP2, and inactivation of the ERK\/MAPK pathway. In conclusion, we found that the up\u2010regulation of UGDH is related to ovarian cancer metastasis and the deficiency of UGDH leads to the decrease of cell migration, cell invasion, wound healing and cell proliferation ability. Our findings reveal that UGDH can serve as a prognostic marker and that the inhibition of UGDH is a promising strategy for ovarian cancer treatment.<\/jats:p>","DOI":"10.1111\/jcmm.15808","type":"journal-article","created":{"date-parts":[[2020,9,8]],"date-time":"2020-09-08T13:07:07Z","timestamp":1599570427000},"page":"11883-11902","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Targeting UDP\u2010glucose dehydrogenase inhibits ovarian cancer growth and metastasis"],"prefix":"10.1111","volume":"24","author":[{"given":"Li\u2010Hsun","family":"Lin","sequence":"first","affiliation":[{"name":"Institute of Bioinformatics and Structural Biology National Tsing Hua University Hsinchu Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsiu\u2010Chuan","family":"Chou","sequence":"additional","affiliation":[{"name":"Institute of Analytical and Environmental Sciences National Tsing Hua University Hsinchu 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