{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T21:54:53Z","timestamp":1771365293194,"version":"3.50.1"},"reference-count":43,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T00:00:00Z","timestamp":1598918400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81774000"],"award-info":[{"award-number":["81774000"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Selaginella moellendorffii<\/jats:italic> Hieron. (SM), a perennial evergreen plant, has been used in the treatment of acute infectious hepatitis, thoracic and hypochondriac lumbar contusions, systemic oedema and thrombocytopaenia. However, the role of a biflavonoid\u2010rich extract from SM (SM\u2010BFRE) in anti\u2010larynx cancer has rarely been reported. In this study, the in vitro and in vivo anti\u2010laryngeal cancer activity and potential mechanisms of SM\u2010BFRE were investigated. An off\u2010line semipreparative liquid chromatography\u2010nuclear magnetic resonance protocol was carried out to determine six biflavonoids from SM\u2010BFRE. In vitro, MTT assay revealed that SM\u2010BFRE inhibited the proliferation of laryngeal carcinoma cells. A wound healing assay indicated that SM\u2010BFRE suppressed the migration of laryngeal cancer cells. Hoechst 33\u00a0258 and Annexin V\u2010FITC\/PI double staining assays were performed and verified that SM\u2010BFRE induced apoptosis in laryngeal carcinoma cells. The Hep\u20102 bearing nude mouse model confirmed that SM\u2010BFRE also exhibited anticancer effect in vivo. In addition, Western blot analysis demonstrated that SM\u2010BFRE exerted its anti\u2010laryngeal cancer effect by activating the mitochondrial apoptotic pathway and inhibiting STAT3 and Akt\/NF\u2010\u03baB signalling pathways. All results suggested that SM\u2010BFRE could be considered as a potential chemotherapeutic drug for laryngeal cancer.<\/jats:p>","DOI":"10.1111\/jcmm.15812","type":"journal-article","created":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T11:42:26Z","timestamp":1598960546000},"page":"11922-11935","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["A biflavonoid\u2010rich extract from <i>Selaginella moellendorffii<\/i> Hieron. induces apoptosis via STAT3 and Akt\/NF\u2010\u03baB signalling pathways in laryngeal carcinoma"],"prefix":"10.1111","volume":"24","author":[{"given":"Huiqi","family":"Huang","sequence":"first","affiliation":[{"name":"School of Pharmaceutical Sciences South\u2010Central University for Nationalities Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji","family":"Hao","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences South\u2010Central 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