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Inflammation due to wear particles and osteoclast activation are the key factors in osteolysis and are also potential targets for the treatment of osteolysis. However, it is not clear whether puerarin can inhibit chronic inflammation and alleviate osteolysis. In this study, we investigated the effect of puerarin on Ti particle\u2010induced inflammatory osteolysis in vivo in rat femoral models and in vitro in receptor activator of nuclear factor kappa\u2010B ligand (RANKL)\u2010induced osteoclast activation models. Our in vivo results showed that puerarin significantly inhibited Ti particle\u2010induced osteolysis and the expression of matrix metallopeptidase 9 (MMP\u20109), nuclear factor of activated T cells 1 (NFATc1), tumour necrosis factor (TNF)\u2010\u03b1 and interleukin (IL)\u20106. In vitro, puerarin prevented RANKL\u2010induced osteoclast differentiation, bone resorption and F\u2010actin ring formation in a concentration\u2010dependent manner. Furthermore, puerarin decreased the phosphorylation of p65 and prevented p65 moving from the cytoplasm to the nucleus. Puerarin also reduced the expression of osteoclast\u2010specific factors and inhibited the inflammatory response. In conclusion, our study proves that puerarin can block the NF\u2010\u03baB signalling pathway to inhibit osteoclast activation and inflammatory processes, which provides a new direction for the treatment of osteolysis\u2010related diseases.<\/jats:p>","DOI":"10.1111\/jcmm.15821","type":"journal-article","created":{"date-parts":[[2020,9,17]],"date-time":"2020-09-17T07:20:23Z","timestamp":1600327223000},"page":"11972-11983","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Puerarin inhibits titanium particle\u2010induced osteolysis and RANKL\u2010induced osteoclastogenesis via suppression of the NF\u2010\u03baB signaling pathway"],"prefix":"10.1111","volume":"24","author":[{"given":"Wenkai","family":"Tang","sequence":"first","affiliation":[{"name":"Department of Orthopedics Zhangjiagang TCM Hospital Affiliated to Nanjing University of Chinese Medicine  Zhangjiagang 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