{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T12:59:26Z","timestamp":1789131566955,"version":"build-2803163510"},"reference-count":36,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T00:00:00Z","timestamp":1598918400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Neuroimmune interactions are involved in the development of endometriosis. Here, we examined the role of a neuropeptide, calcitonin gene\u2013related peptide (CGRP), and its receptor, receptor activity\u2013modifying protein (RAMP) 1, in growth of endometrial tissues and the formation of blood and lymphatic vessels in a mouse ectopic endometrial transplantation model. Endometrial fragments from donor wild\u2010type (WT) mice transplanted into the peritoneal wall of recipient WT mice grew with increased density of blood and lymphatic vessels. When tissues from RAMP1\u2010deficient (RAMP1<jats:sup>\u2212\/\u2212<\/jats:sup>) mice were transplanted into RAMP1<jats:sup>\u2212\/\u2212<\/jats:sup> mice, implant growth and angiogenesis\/lymphangiogenesis were decreased. CGRP was up\u2010regulated in dorsal root ganglia, and CGRP<jats:sup>+<\/jats:sup> nerve fibres were distributed into the implants from the peritoneum. RAMP1 was co\u2010expressed with CD11b (macrophages) and S100A4 (fibroblasts), but did not co\u2010localize with blood vessel endothelial cell marker CD31 or lymphatic vessel endothelial hyaluronan receptor (LYVE)\u20101. Cultured with CGRP, macrophages up\u2010regulated vascular endothelial growth factor (VEGF)\u2010A, VEGF\u2010C and VEGF\u2010D, whereas fibroblasts up\u2010regulated VEGF\u2010C, but not VEGF\u2010A or VEGF\u2010D, in a RAMP1\u2010dependent manner. CGRP receptor antagonist CGRP8\u201037 inhibited growth of and angiogenesis\/lymphangiogenesis within endometrial tissue implants. These results suggest that RAMP1 signalling is crucial for growth and angiogenesis\/lymphangiogenesis in endometrial tissue. Blockade of RAMP1 is a potential tool for the treatment of endometriosis.<\/jats:p>","DOI":"10.1111\/jcmm.15823","type":"journal-article","created":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T11:22:36Z","timestamp":1598959356000},"page":"11984-11997","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Inhibition of receptor activity\u2013modifying protein 1 suppresses the development of endometriosis and the formation of blood and lymphatic vessels"],"prefix":"10.1111","volume":"24","author":[{"given":"Masako","family":"Honda","sequence":"first","affiliation":[{"name":"Department of Pharmacology Kitasato University School of Medicine Sagamihara Japan"},{"name":"Department of Molecular Pharmacology Graduate School of Medical Sciences Sagamihara Japan"},{"name":"Department of Obstetrics and Gynecology Graduate School of Medical Sciences Sagamihara 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