{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,25]],"date-time":"2026-10-25T11:53:58Z","timestamp":1792929238898,"version":"4.5.0"},"reference-count":42,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2020,9,20]],"date-time":"2020-09-20T00:00:00Z","timestamp":1600560000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81572420"],"award-info":[{"award-number":["81572420"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We previously demonstrated that cancer\u2010associated fibroblasts (CAFs) promoted the proliferation of gallbladder cancer (GBC) cells, but the mechanism is not clear. Neuropilin\u20101 (<jats:italic>NRP\u20101<\/jats:italic>) plays an important role in various malignancies as transmembrane glycoprotein. Our goal was to reveal the relationship between CAFs and <jats:italic>NRP\u20101<\/jats:italic> and their potential functions in GBC. In this study, we found <jats:italic>NRP\u20101<\/jats:italic> was overexpressed in GBC tissue, associated with poor survival and was up\u2010regulated by CAFs. The cytokine array cluster analysis revealed IL\u20108 secreted by CAFs facilitated the up\u2010regulation of <jats:italic>NRP\u20101<\/jats:italic> in tumour cells. <jats:italic>NRP\u20101<\/jats:italic> knockdown suppressed tumour growth in vivo. Gene expression microarray analysis showed 581 differentially regulated genes under <jats:italic>NRP\u20101<\/jats:italic> knockdown conditions. Ingenuity pathway analysis demonstrated that <jats:italic>NRP\u20101<\/jats:italic> knockdown may inhibit tumour progression by affecting cell proliferation. We then confirmed that <jats:italic>NRP\u20101<\/jats:italic> knockdown in NOZ and GBC\u2010SD cells significantly inhibited cell proliferation. Additionally, the IL\u20108 mediated MDM2 and CCNA2 expression were affected by <jats:italic>NRP\u20101<\/jats:italic> knockdown. Our findings suggested that <jats:italic>NRP\u20101<\/jats:italic> was up\u2010regulated by CAF\u2010secreted IL\u20108, which subsequently promoted GBC cell proliferation, and these molecules may serve as useful prognostic biomarkers and therapeutic targets for GBC.<\/jats:p>","DOI":"10.1111\/jcmm.15825","type":"journal-article","created":{"date-parts":[[2020,9,20]],"date-time":"2020-09-20T09:25:32Z","timestamp":1600593932000},"page":"12608-12618","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Neuropilin\u20101 is up\u2010regulated by cancer\u2010associated fibroblast\u2010secreted IL\u20108 and associated with cell proliferation of gallbladder cancer"],"prefix":"10.1111","volume":"24","author":[{"given":"Chen","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Hepatobiliary Surgery The First Affiliated Hospital of Xi\u2019an Jiaotong University  Xi\u2019an Shaanxi Province 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