{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T11:40:11Z","timestamp":1771501211190,"version":"3.50.1"},"reference-count":15,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,2,1]],"date-time":"2021-02-01T00:00:00Z","timestamp":1612137600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The over\u2010activation of inflammation is involved in the pathogenesis of smoke\u2010induced lung injury (SILI), while Rb3 treatment may alleviate smoke\u2010induced lung injury by down\u2010regulating the expression of H19, a regulator of miR\u201029b expression. Moreover, HMGB1 is an important mediator of inflammation. Therefore, in this study, we set up an animal model of SILI and treated it with Rb3 to study the effect of Rb3 on the treatment of SILI and the involvement of H19\/miR\u201029b\/HMGB1\/TLR4 signalling. SILI mice treated with Rb3 before H&amp;E staining and TUNEL assay were conducted to observe the pathological damages and status of apoptosis in each group. Real\u2010time PCR, Western blot, computational analysis and luciferase assays were utilized to establish the signalling pathway involved in the pathogenesis of SILI and the action of Rb3 treatment. Rb3 treatment alleviated pathological changes in the lungs while decreasing the levels of W\/D ratio and cell apoptotic index. H19 was validated to sponge miR\u201029b\u20103p, while HMGB1 mRNA was validated to be a target gene of miR\u201029b\u20103. As a result, a signalling pathway of H19\/miR\u201029b\u20103p\/HMGB1 was established. Cell viability was evidently reduced after 72\u00a0hours of treatment with CSE, but the treatment of Rb3 elevated the expression of H19 and HMBG1 in the presence of CSE. Also, CSE\u2010induced inhibition of miR\u201029b\u20103p expression was restored by Rb3. The findings of this study collectively demonstrated that Rb3 exhibited its therapeutic effect during the treatment of SILI via modulating the H19\/miR\u201029b\u20103p\/HMBG1 signalling pathway.<\/jats:p>","DOI":"10.1111\/jcmm.15844","type":"journal-article","created":{"date-parts":[[2021,2,2]],"date-time":"2021-02-02T06:34:08Z","timestamp":1612247648000},"page":"2725-2729","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Ginsenoside Rb3 alleviates smoke\u2010induced lung injury via the H19\/miR\u201029b\u20103p\/HGMB1\/TLR4 signalling pathway"],"prefix":"10.1111","volume":"25","author":[{"given":"Yan","family":"Tan","sequence":"first","affiliation":[{"name":"Department of Pulmonary and Critical Care Medicine The First People\u2019s Hospital of Yunnan Province Kunming China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danxiong","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Pulmonary and Critical Care Medicine The First People\u2019s Hospital of Yunnan Province Kunming China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pulmonary and Critical Care Medicine The First People\u2019s Hospital of Yunnan Province Kunming China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rufang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pulmonary and Critical Care Medicine The First People\u2019s Hospital of Yunnan Province Kunming China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2154-9241","authenticated-orcid":false,"given":"Shenglan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pulmonary and Critical Care Medicine The First People\u2019s Hospital of Yunnan Province Kunming China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,2]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpi.12192"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2016.10.029"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.180.2.1169"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.10.018"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1159\/000495552"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2017.00976"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.285.5425.248"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-018-1219-0"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/1745-6215-12-164"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.23380"},{"key":"e_1_2_10_12_1","first-page":"557","article-title":"Micro\u2010ribonucleic acid 29b inhibits cell proliferation and invasion and enhances cell apoptosis and chemotherapy effects of cisplatin via targeting of DNMT3b and AKT3 in prostate cancer","volume":"8","author":"Yan B","year":"2015","journal-title":"Onco Targets Ther"},{"issue":"3","key":"e_1_2_10_13_1","first-page":"929","article-title":"lncRNA H19 promotes viability and epithelial\u2010mesenchymal transition of lung adenocarcinoma cells by targeting miR\u201029b\u20103p and modifying STAT3","volume":"54","author":"Liu L","year":"2019","journal-title":"Int J Oncol"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni1457"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2011.08.012"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-011-1106-6"}],"container-title":["Journal of Cellular and Molecular 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