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In this study, we aimed to elucidate the mechanisms of MSCs\u2010extracellular vesicles (EVs) carrying transforming growth factor\u2010beta 1 (TGF\u2010\u03b21) in M2 polarization in mouse macrophages via the microRNA\u2010132 (miR\u2010132)\/E3 ubiquitin ligase myc binding protein 2 (Mycbp2)\/tuberous sclerosis complex 2 (TSC2) axis. Mouse MSCs were isolated for adipogenic and osteogenic induction, followed by co\u2010culture with mouse macrophages RAW264.7. Besides, mouse macrophages RAW264.7 were co\u2010cultured with MSCs\u2010EVs in vitro, where the proportion of macrophages and inflammation were detected by flow cytometry and ELISA. The experimental data revealed that MSCs\u2010EVs promoted M2 polarization of macrophages, and elevated interleukin (IL)\u201010 expression and inhibited levels of IL\u20101\u03b2, tumour necrosis factor (TNF)\u2010\u03b1 and IL\u20106. MSC\u2010EV\u2010treated macrophages RAW264.7 increased TGF\u2010\u03b21 expression, thus elevating miR\u2010132 expression. MiR\u2010132 directly bound to Mycbp2, as confirmed by luciferase activity assay. Meanwhile, E3 ubiquitin ligase Mycbp2 could ubiquitinate TSC2 protein. Furthermore, silencing TGF\u2010\u03b21 inhibited M2 polarization of MSC\u2010EV\u2010treated macrophages. Taken conjointly, this study provides evidence reporting that MSC\u2010secreted EVs carry TGF\u2010\u03b21 to promote M2 polarization of macrophages via modulation of the miR\u2010132\/Mycbp2\/TSC2 axis.<\/jats:p>","DOI":"10.1111\/jcmm.15860","type":"journal-article","created":{"date-parts":[[2020,9,23]],"date-time":"2020-09-23T13:44:52Z","timestamp":1600868692000},"page":"12750-12764","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":59,"title":["Mesenchymal stem cell\u2013secreted extracellular vesicles carrying TGF\u2010\u03b21 up\u2010regulate miR\u2010132 and promote mouse M2 macrophage polarization"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7889-2262","authenticated-orcid":false,"given":"Yongqi","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Anesthesiology the First Hospital of Lanzhou University Lanzhou 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