{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T22:04:57Z","timestamp":1772834697364,"version":"3.50.1"},"reference-count":22,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,11,17]],"date-time":"2020-11-17T00:00:00Z","timestamp":1605571200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Osteoarthritis (OA) is an ageing\u2010related disease characterized by articular cartilage degradation and joint inflammation. circRNA has been known to involve in the regulation of multiple inflammatory diseases including OA. However, the mechanism underlying how circRNA regulates OA remains to be elucidated. Here, we report circANKRD36 prevents OA chondrocyte apoptosis and inflammation by targeting miR\u2010599, which specifically degrades Casz1. We performed circRNA sequencing in normal and OA tissues and found the expression of circANKRD36 is decreased in OA tissues. circANKRD36 is also reduced in IL\u20101\u03b2\u2013treated human chondrocytes. FACS analysis and Western blot showed that the knockdown of circANKRD36 promotes the apoptosis and inflammation of chondrocytes in IL\u20101\u03b2 stress. We then found miR\u2010599 to be the target of circANKRD36 and correlate well with circANKRD36 both in vitro and in vivo. By database analysis and luciferase assay, Casz1 was found to be the direct target of miR\u2010599. Casz1 helps to prevent apoptosis and inflammation of chondrocytes in response to IL\u20101\u03b2. In conclusion, our results proved circANKRD36 sponge miR\u2010599 to up\u2010regulate the expression of Casz1 and thus prevent apoptosis and inflammation in OA.<\/jats:p>","DOI":"10.1111\/jcmm.15884","type":"journal-article","created":{"date-parts":[[2020,11,18]],"date-time":"2020-11-18T07:00:21Z","timestamp":1605682821000},"page":"120-131","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Circular RNA circANKRD36 regulates Casz1 by targeting miR\u2010599 to prevent osteoarthritis chondrocyte apoptosis and inflammation"],"prefix":"10.1111","volume":"25","author":[{"given":"Jian\u2010Lin","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Orthopedics Renmin Hospital of Wuhan University Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuang","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Renmin Hospital of Wuhan University Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong\u2010Song","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Renmin Hospital of Wuhan University Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xian\u2010jin","family":"Du","sequence":"additional","affiliation":[{"name":"Department of Emergency Renmin Hospital of Wuhan University Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Renmin Hospital of Wuhan University Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8501-0878","authenticated-orcid":false,"given":"Qiong\u2010jie","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Radiology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,11,17]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2014.162"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2016.72"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2018.07.019"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/boneres.2016.44"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2015.10.018"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2016.119"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.03.008"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12986"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2013.53"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature11993"},{"issue":"4","key":"e_1_2_8_12_1","first-page":"1865","article-title":"Screening of circular RNAs and validation of circANKRD36 associated with inflammation in patients with type 2 diabetes mellitus","volume":"42","author":"Fang Y","year":"2018","journal-title":"Int J Mol Med"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(92)90234-5"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2013.03.003"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2010.187"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.00184"},{"issue":"8","key":"e_1_2_8_17_1","first-page":"523","article-title":"Osteoarthritis: rapid evidence review","volume":"97","author":"Ebell MH","year":"2018","journal-title":"Am Fam Physician"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1042\/BSR20180542"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.3380"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12950-020-0234-y"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.29490"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.29154"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/1440-1681.13316"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.15884","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jcmm.15884","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.15884","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,3]],"date-time":"2023-09-03T08:10:22Z","timestamp":1693728622000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.15884"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,11,17]]},"references-count":22,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,1]]}},"alternative-id":["10.1111\/jcmm.15884"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.15884","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,11,17]]},"assertion":[{"value":"2020-04-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-08-28","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-11-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}