{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T23:14:56Z","timestamp":1769728496434,"version":"3.49.0"},"reference-count":26,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2020,10,13]],"date-time":"2020-10-13T00:00:00Z","timestamp":1602547200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>MicroRNAs (miRNAs) are emerging biomarkers in biological processes and the role of miR\u2010495\u20103p has been identified in melanoma, while the detailed molecular mechanisms remain to be further explored. We aim to explore the effect of histone deacetylase 3 (HDAC3) and miR\u2010495\u20103p on epithelial\u2010mesenchymal transition (EMT) and oncogenicity of melanoma cells by regulating tumour necrosis factor receptor\u2010associated factor 5 (TRAF5). Levels of HDAC3, miR\u2010495\u20103p and TRAF5 in melanoma tissues and pigmented nevus tissues were determined, and the predictive roles of HDAC3 and miR\u2010495\u20103p in prognosis of melanoma patients were measured. The melanoma cells were screened and transfected with relative oligonucleotides and plasmids, and the expression of HDAC3, miR\u2010495\u20103p and TRAF5, and phenotypes of melanoma cells were gauged by a series of assays. The relations between HDAC3 and miR\u2010495\u20103p, and between miR\u2010495\u20103p and TRAF5 were confirmed. HDAC3 and TRAF5 were increased while miR\u2010495\u20103p was decreased in melanoma cells and tissues, and the low expression of miR\u2010495\u20103p as well as high expression of HDAC3 indicated a poor prognosis of melanoma patients. Inhibited HDAC3 elevated miR\u2010495\u20103p to suppress EMT and oncogenicity of melanoma cells by reducing TRAF5. HDAC3 particularly bound to miR\u2010495\u20103p and TRAF5 was the target gene of miR\u2010495\u20103p. Our results revealed that down\u2010regulated HDAC3 elevates miR\u2010495\u20103p to suppress malignant phenotypes of melanoma cells by inhibiting TRAF5, thereby repressing EMT progression of melanoma cells. This study may provide novel targets for melanoma treatment.<\/jats:p>","DOI":"10.1111\/jcmm.15885","type":"journal-article","created":{"date-parts":[[2020,10,13]],"date-time":"2020-10-13T19:02:58Z","timestamp":1602615778000},"page":"12933-12944","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Down\u2010regulated HDAC3 elevates microRNA\u2010495\u20103p to restrain epithelial\u2010mesenchymal transition and oncogenicity of melanoma cells via reducing TRAF5"],"prefix":"10.1111","volume":"24","author":[{"given":"Yanbo","family":"Ma","sequence":"first","affiliation":[{"name":"Oral and maxillofacial surgery Linyi People's Hospital Linyi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jincheng","family":"Duan","sequence":"additional","affiliation":[{"name":"Department of stomatology Linyi People's Hospital Linyi 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