{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T10:40:21Z","timestamp":1782816021628,"version":"3.54.5"},"reference-count":59,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2020,9,25]],"date-time":"2020-09-25T00:00:00Z","timestamp":1600992000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81903105"],"award-info":[{"award-number":["81903105"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Ebselen, an organo\u2010selenium compound with well\u2010characterized toxicology and pharmacology, recently exhibited potent antibacterial activity against glutathione (GSH)\u2010negative bacteria by disrupting redox homeostasis. In this paper, we show that ebselen and silver ion in combination exert strong bactericidal activity against multidrug\u2010resistant (MDR) uropathogenic <jats:italic>Escherichia coli<\/jats:italic> (UPEC) BC1, a model MDR GSH\u2010positive bacterium. The mechanisms were found to involve consumption of total intracellular GSH and inhibition of thioredoxin reductase activity, which was highly related to reactive oxygen species up\u2010regulation. Furthermore, the therapeutic efficacy of ebselen and silver ion against UPEC\u2010induced cystitis was assessed in a mouse model. Treatment with ebselen and silver ion significantly reduced bacterial loads, down\u2010regulated the expression levels of tumour necrosis factor\u2010\u03b1 (TNF\u2010\u03b1) and interferon\u2010\u03b3 (IFN\u2010\u03b3) on\u2010site and decreased white\/red blood cell counts in mild cystitis model mice, which demonstrated the anti\u2010inflammatory property of these agents. In addition, ebselen and silver ion also exhibited significantly high protective ability (100%) against acute cystitis infections. These results together may lay the foundation for further analysis and development of ebselen and silver ion as antibacterial agents for treatment of MDR UPEC infections.<\/jats:p>","DOI":"10.1111\/jcmm.15920","type":"journal-article","created":{"date-parts":[[2020,9,25]],"date-time":"2020-09-25T11:31:37Z","timestamp":1601033497000},"page":"13139-13150","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Depletion of multidrug\u2010resistant uropathogenic <i>Escherichia coli<\/i> BC1 by ebselen and silver ion"],"prefix":"10.1111","volume":"24","author":[{"given":"Peng","family":"Wang","sequence":"first","affiliation":[{"name":"The First College of Clinical Medical Science China Three Gorges University Yichang, Hubei China"},{"name":"The Institute of Infection and Inflammation Medical College China Three Gorges University Yichang, Hubei China"},{"name":"Central Laboratory The People\u2019s Hospital of China Three Gorges University Yichang, Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2343-4381","authenticated-orcid":false,"given":"Jun","family":"Wang","sequence":"additional","affiliation":[{"name":"The Institute of Infection and Inflammation Medical College China Three Gorges University Yichang, Hubei China"},{"name":"Central Laboratory The People\u2019s Hospital of China Three Gorges University Yichang, Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zonglan","family":"Xie","sequence":"additional","affiliation":[{"name":"The First College of Clinical Medical Science China Three Gorges University Yichang, Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingxuan","family":"Zhou","sequence":"additional","affiliation":[{"name":"The Institute of Infection and Inflammation Medical College China Three Gorges University Yichang, Hubei China"},{"name":"Central Laboratory The 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