{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:10:30Z","timestamp":1787015430533,"version":"build-2736575974"},"reference-count":63,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2020,10,13]],"date-time":"2020-10-13T00:00:00Z","timestamp":1602547200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008804","name":"Ministry of Science and Technology, Government of the People\u2019s Republic of Bangladesh","doi-asserted-by":"publisher","award":["39.009.002.01.00.057.2015\u20102016\/922\/Phys\u2010362"],"award-info":[{"award-number":["39.009.002.01.00.057.2015\u20102016\/922\/Phys\u2010362"]}],"id":[{"id":"10.13039\/501100008804","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The importance of biogenic silver\/silver chloride nanoparticles has become increasing day by day. In the present study, silver\/silver chloride nanoparticles (Ag\/AgCl\u2010NPs) were synthesized from\n                    <jats:italic>Kaempferia rotunda<\/jats:italic>\n                    tuberous rhizome extract to evaluate the antiproliferative activity against human glioblastoma stem cells (GSCs) in vitro and Ehrlich ascites carcinoma (EAC) cells in vivo in mice. Synthesis of nanoparticles was confirmed by colour change and UV\u2010visible spectrum and characterized by TEM, XRD, TGA, AFM and FTIR.\n                    <jats:italic>K rotunda<\/jats:italic>\n                    and recently synthesized\n                    <jats:italic>Zizyphus mauritiana<\/jats:italic>\n                    fruit extract\u2010mediated Ag\/AgCl\u2010NPs inhibited 77.2% and 71% of GSCs growth at 32 \u00b5g\/mL concentration with the IC\n                    <jats:sub>50<\/jats:sub>\n                    values of 6.8 and 10.4\u00a0\u00b5g\/mL, respectively. Cell morphological studies and caspase\u20103 immunofluorescence assay revealed that both biogenic nanoparticles induced apoptosis in GSCs. Expression levels of several genes were checked by real\u2010time PCR after treatment with\n                    <jats:italic>K rotunda<\/jats:italic>\n                    tuberous rhizome\u2010mediated Ag\/AgCl\u2010NPs. PARP, EGFR, NOTCH2 and STAT3 gene expression were decreased with the increase of NF\u03baB, TLR9, IL1, TNF\u03b1, IKK and p21 gene that would be the cause of induction of apoptosis in GSCs. The cell cycle arrest at G\n                    <jats:sub>2<\/jats:sub>\n                    \/M phase was confirmed by flow cytometric assay. Both nanoparticles were injected intraperitoneally to rapidly growing EAC cells for 5 consecutive days. Approximately, 32.3% and 55% EAC cells growth were inhibited by\n                    <jats:italic>K rotunda<\/jats:italic>\n                    tuberous rhizome\u2010mediated Ag\/AgCl\u2010NPs at 6 and 12\u00a0mg\/kg\/day doses, respectively while only 20% cell growth inhibition was monitored at 12\u00a0mg\/kg\/day dose of\n                    <jats:italic>Z mauritiana<\/jats:italic>\n                    <jats:bold>\u2010<\/jats:bold>\n                    mediated Ag\/AgCl\u2010NPs. From the above results, it can be concluded that presently synthesized nanoparticles would be a potent anticancer agent.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.15934","type":"journal-article","created":{"date-parts":[[2020,10,13]],"date-time":"2020-10-13T10:21:51Z","timestamp":1602584511000},"page":"13223-13234","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Biogenic silver\/silver chloride nanoparticles inhibit human glioblastoma stem cells growth in vitro and Ehrlich ascites carcinoma cell growth in vivo"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5708-014X","authenticated-orcid":false,"given":"Syed Rashel","family":"Kabir","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology University of Rajshahi Rajshahi Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi","family":"Dai","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences, Kunming Institute of Zoology Kunming Yunnan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Nurujjaman","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology University of Rajshahi Rajshahi Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyue","family":"Cui","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences, Kunming Institute of Zoology Kunming Yunnan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. 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