{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T17:33:29Z","timestamp":1787852009696,"version":"build-2784847793"},"reference-count":33,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2020,10,8]],"date-time":"2020-10-08T00:00:00Z","timestamp":1602115200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Aster tataricus<\/jats:italic> L.f. is a traditional Eastern Asian herbal medicine used for the relief of uroschesis\u2010related illnesses and has been demonstrated clinically to exert satisfied effects. However, the mechanism of its therapeutic action remains unclear. The present study aimed to evaluate the protective mechanism of <jats:italic>Aster tataricus<\/jats:italic> extract (ATE) on CYP or LPS\u00a0+\u00a0ATP\u2010induced interstitial cystitis (IC), we successfully constructed the induced IC Sprague\u2010Dawley (SD) rat model and IC human urothelium cell (SV\u2010HUC\u20101) model. The main compounds of ATE were determined by LC\u2010MS. After intervention, the changes on the bladder wall morphology and inflammation were observed in each group. SV\u2010HUC1 cell viability was measured by MTT and double stained with Hoechst 33342 and propidium iodide (PI). The expression levels of NLRP3, Pro\u2010caspase\u20101, Caspsae\u20101 p20, GSDMD, GSDMD\u2010N and Cleave\u2010IL\u20101\u03b2 in vivo and in vitro in different groups were detected by Western blotting. ATE significantly alleviated oedema and haemorrhage and reduced the inflammation index and histopathological score in SD rat bladder. The results of cell revealed that ATE could improve cell viability and decrease pyroptosis ratio. The expression of NLRP3 and other pyroptosis\u2010related protein was remarkably decreased by ATE both in vivo and in vitro. ATE may be used as an inhibitor of NLRP3 in treating IC. The discovery of NLRP3\/Caspase\u20101\/GSDMD\u2010N as a new protective pathway provides a new direction for protecting cell against IC.<\/jats:p>","DOI":"10.1111\/jcmm.15952","type":"journal-article","created":{"date-parts":[[2020,10,8]],"date-time":"2020-10-08T09:16:41Z","timestamp":1602148601000},"page":"13336-13345","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Protective effect of <i>Aster tataricus<\/i> extract on NLRP3\u2010mediated pyroptosis of bladder urothelial cells"],"prefix":"10.1111","volume":"24","author":[{"given":"Xin","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Nephrology Suzhou Hospital of Integrated Traditional Chinese and Western Medicine Suzhou China"},{"name":"Li Shicai School Inheritance Studio Suzhou Hospital of Integrated Traditional Chinese and Western Medicine Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Suzhou Hospital of Integrated Traditional Chinese and Western Medicine Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of Nephrology Suzhou Hospital of Integrated Traditional Chinese and Western Medicine Suzhou China"},{"name":"Li Shicai School Inheritance Studio Suzhou Hospital of Integrated Traditional Chinese and Western Medicine Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiqun","family":"Zhou","sequence":"additional","affiliation":[{"name":"Li Shicai School Inheritance Studio Suzhou Hospital of Integrated Traditional Chinese and Western Medicine Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolong","family":"Tang","sequence":"additional","affiliation":[{"name":"Li Shicai School Inheritance Studio Suzhou Hospital of Integrated Traditional Chinese and Western Medicine Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojun","family":"Fei","sequence":"additional","affiliation":[{"name":"Li Shicai School Inheritance Studio Suzhou Hospital of Integrated Traditional Chinese and Western Medicine Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hailin","family":"Tang","sequence":"additional","affiliation":[{"name":"Li Shicai School Inheritance Studio Suzhou Hospital of Integrated Traditional Chinese and Western Medicine Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Peng","sequence":"additional","affiliation":[{"name":"Li Shicai School Inheritance Studio Suzhou Hospital of Integrated Traditional Chinese and Western Medicine Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoqin","family":"Ren","sequence":"additional","affiliation":[{"name":"Li Shicai School Inheritance Studio Suzhou Hospital of Integrated Traditional Chinese and Western Medicine Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Xue","sequence":"additional","affiliation":[{"name":"Department of Nephrology Suzhou Hospital of Integrated Traditional Chinese and Western Medicine Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunli","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Nephrology Suzhou Hospital of Integrated Traditional Chinese and Western Medicine Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianping","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Nephrology Suzhou Hospital of Integrated Traditional Chinese and Western Medicine Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinglei","family":"Jin","sequence":"additional","affiliation":[{"name":"Li Shicai School Inheritance Studio Suzhou Hospital of Integrated Traditional Chinese and Western Medicine Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7500-2591","authenticated-orcid":false,"given":"Qingjiang","family":"Jin","sequence":"additional","affiliation":[{"name":"Li Shicai School Inheritance Studio Suzhou Hospital of Integrated Traditional Chinese and Western Medicine Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,10,8]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2020.01.002"},{"key":"e_1_2_9_3_1","article-title":"A systematic review of surgical interventions for the treatment of bladder pain syndrome\/interstitial cystitis","author":"Osman NI","year":"2020","journal-title":"Eur Urol Focus"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2018.02.039"},{"key":"e_1_2_9_5_1","article-title":"Mechanisms underlying overactive bladder and interstitial cystitis\/painful bladder syndrome","volume":"12","author":"Grundy L","year":"2018","journal-title":"Front Neurosci\u2010Switz"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/1756287218818030"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2019.153111"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2020.03.007"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2019.103208"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2015.02.036"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1532-0456(03)00170-4"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2017.01.179"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apjtm.2016.07.012"},{"key":"e_1_2_9_14_1","first-page":"211","article-title":"Hyperbaric oxygen treatment time for cyclophosphamide induced cystitis in rats","volume":"31","author":"Oztas E","year":"2004","journal-title":"Undersea Hyperbar M"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(17)44929-9"},{"key":"e_1_2_9_16_1","doi-asserted-by":"crossref","first-page":"4206","DOI":"10.1074\/jbc.M109.040444","article-title":"Endogenous NGF regulates collagen expression and bladder hypertrophy through Akt and MAPK pathways during cystitis","volume":"285","author":"Chung C","year":"2009","journal-title":"J Biol Chem"},{"key":"e_1_2_9_17_1","first-page":"680","article-title":"Losartan inhibits LPS + ATP\u2010induced IL\u20101beta secretion from mouse primary macrophages by suppressing NALP3 inflammasome","volume":"69","author":"Wang F","year":"2014","journal-title":"Pharmazie"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2020.117590"},{"key":"e_1_2_9_19_1","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.urology.2015.12.040","article-title":"Inflammation and symptom change in interstitial cystitis or bladder pain syndrome: a multidisciplinary approach to the study of chronic pelvic pain research network study","volume":"90","author":"Schrepf A","year":"2015","journal-title":"Urology"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1177\/1756287211398255"},{"key":"e_1_2_9_21_1","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1016\/S0022-5347(17)41173-6","article-title":"Urological complications of cyclophosphamide","volume":"141","author":"Levine LA","year":"1989","journal-title":"Urology"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scr.2020.101801"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcp.2020.101596"},{"key":"e_1_2_9_24_1","doi-asserted-by":"crossref","DOI":"10.1111\/acer.14338","article-title":"Inhibition of HSP90 and activation of HSF1 diminishes macrophage NLRP3 inflammasome activity in alcoholic liver injury","volume":"44","author":"Choudhury A","year":"2020","journal-title":"Alcohol Clin Exp Res"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2020.02.149"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trim.2020.101293"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2017.170244"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.11.047"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature15514"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0305-7372(83)80002-4"},{"key":"e_1_2_9_31_1","first-page":"109","article-title":"Study on the defecated and diuretic function of Ziwan","volume":"28","author":"Jia ZX","year":"2012","journal-title":"Pharm Clin Chin Materia Medica"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/chromsci\/bmv171"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00011-019-01241-z"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2020.117901"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.15952","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jcmm.15952","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.15952","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,4]],"date-time":"2023-09-04T14:38:22Z","timestamp":1693838302000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.15952"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10,8]]},"references-count":33,"journal-issue":{"issue":"22","published-print":{"date-parts":[[2020,11]]}},"alternative-id":["10.1111\/jcmm.15952"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.15952","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,10,8]]},"assertion":[{"value":"2020-07-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-08-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-10-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}