{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T16:42:29Z","timestamp":1790959349631,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,11,21]],"date-time":"2020-11-21T00:00:00Z","timestamp":1605916800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81900334"],"award-info":[{"award-number":["81900334"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>miRNA\u2010mediated pyroptosis play crucial effects in the development of myocardial ischaemia\/reperfusion (I\/R) injury (MIRI). Piperine (PIP) possesses multiple pharmacological effects especially in I\/R condition. This study focuses on whether PIP protects MIRI from pyroptosis via miR\u2010383\u2010dependent pathway. Rat MIRI model was established by 30\u00a0minutes of LAD ligation and 4\u00a0hours of reperfusion. Myocardial enzymes, histomorphology, structure and function were detected to evaluate MIRI. Recombinant adenoviral vectors for miR\u2010383 overexpression or miR\u2010383 silencing or RP105 knockdown were constructed, respectively. Luciferase reporter analysis was used to confirm RP105 as a target of miR\u2010383. Pyroptosis\u2010related markers were measured by Western blotting assay. The results showed that I\/R provoked myocardial injury, as shown by the increases of LDH\/CK releases, infarcted areas and apoptosis as well as worsened function and structure. Pyroptosis\u2010related mediators including NLRP3, cleaved caspase\u20101, cleaved IL\u20101\u03b2 and IL\u201018 were also reinforced after MIRI. However, PIP treatment greatly ameliorated MIRI in parallel with pyroptotic repression. In mechanistic studies, MIRI\u2010caused elevation of miR\u2010383 and decrease of RP105\/PI3K\/AKT pathway were reverted by PIP treatment. Luciferase reporter assay confirmed RP105 as a miR\u2010383 target. miR\u2010383 knockdown ameliorated but miR\u2010383 overexpression facilitated pyroptosis and MIRI. Moreover, the anti\u2010pyroptotic effect from miR\u2010383 silencing was verified to be relied on the RP105\/PI3K\/AKT signalling pathway. Additionally, our present study further indicated the miR\u2010383\/RP105\/AKT\u2010dependent approach resulting from PIP administration against pyroptosis in MIRI. Therefore, PIP treatment attenuates MIRI and pyroptosis by regulating miR\u2010383\/RP105\/AKT pathway, and it may provide a therapeutic manner for the treatment of MIRI.<\/jats:p>","DOI":"10.1111\/jcmm.15953","type":"journal-article","created":{"date-parts":[[2020,11,21]],"date-time":"2020-11-21T12:48:07Z","timestamp":1605962887000},"page":"244-258","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["Piperine protects against pyroptosis in myocardial ischaemia\/reperfusion injury by regulating the miR\u2010383\/RP105\/AKT signalling pathway"],"prefix":"10.1111","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9479-7756","authenticated-orcid":false,"given":"Xin","family":"Guo","sequence":"first","affiliation":[{"name":"Department of Cardiology The Central Hospital of Wuhan Tongji Medical College Huazhong University of Science and Technology  Wuhan 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