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To further improve the antitumour effect of OA, in this study, we combined CD55\u2010TRAIL\u2010IETD\u2010MnSOD (CD55\u2010TMn), a CEA\u2010controlled OA constructed previously, and chemotherapy to investigate their synergistic effect and possible mechanisms. MTT assay was performed to detect antitumour effects. Hoechst 33\u00a0342 and flow cytometric analysis were used to examine cell apoptosis. Western blotting was performed to examine cell pyroptosis and apoptosis mechanism. Animal experiment was used to detect antitumour effect of doxorubicin hydrochloride (Dox) combined with CD55\u2010TMn in vivo. We firstly found that Dox promotes gene expression mediated by CEA\u2010regulated OA and virus progeny replication by activating phosphorylation of Smad3, and Dox can enhance antitumour effect of CEA\u2010regulated CD55\u2010TMn by promoting cell apotopsis and cell pyroptosis. Thus, our results provide an experimental and theoretical basis on tumour therapy by combination treatment of the oncolytic virotherapy and chemotherapy and it is expected to become a novel strategy for liver cancer therapy.<\/jats:p>","DOI":"10.1111\/jcmm.15966","type":"journal-article","created":{"date-parts":[[2020,10,14]],"date-time":"2020-10-14T12:11:32Z","timestamp":1602677492000},"page":"13431-13439","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Doxorubicin hydrochloride enhanced antitumour effect of CEA\u2010regulated oncolytic virotherapy in live cancer cells and a mouse model"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0564-9944","authenticated-orcid":false,"given":"Boduan","family":"Xiao","sequence":"first","affiliation":[{"name":"Xinyuan Institute of Medicine and Biotechnology School of Life Sciences and Medicine Zhejiang Sci\u2010Tech University Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chang","family":"Ying","sequence":"additional","affiliation":[{"name":"Xinyuan Institute of Medicine and Biotechnology School of Life Sciences and Medicine Zhejiang Sci\u2010Tech University Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6004-1865","authenticated-orcid":false,"given":"Yongyi","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of cancer research and basic medical sciences of Chinese Academy of Sciences Cancer hospital of University of Chinese Academy of Sciences Zhejiang cancer hospital Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fang","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Pathology Zhejiang Provincial People\u2019s Hospital Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Binrong","family":"Wang","sequence":"additional","affiliation":[{"name":"Xinyuan Institute of Medicine and Biotechnology School of Life Sciences and Medicine Zhejiang Sci\u2010Tech University Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huiling","family":"Fang","sequence":"additional","affiliation":[{"name":"Xinyuan Institute of Medicine and Biotechnology School of Life Sciences and Medicine Zhejiang Sci\u2010Tech University Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wan","family":"Guo","sequence":"additional","affiliation":[{"name":"Xinyuan Institute of Medicine and Biotechnology School of Life Sciences and Medicine Zhejiang Sci\u2010Tech University Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tao","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Guangdong General Hospital Guangdong Academy of Medical Sciences Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiumei","family":"Zhou","sequence":"additional","affiliation":[{"name":"Xinyuan Institute of Medicine and Biotechnology School of Life Sciences and Medicine Zhejiang Sci\u2010Tech University Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Biao","family":"Huang","sequence":"additional","affiliation":[{"name":"Xinyuan Institute of Medicine and Biotechnology School of Life Sciences and Medicine Zhejiang Sci\u2010Tech University Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0250-7940","authenticated-orcid":false,"given":"Xinyuan","family":"Liu","sequence":"additional","affiliation":[{"name":"Xinyuan Institute of Medicine and Biotechnology School of Life Sciences and Medicine Zhejiang Sci\u2010Tech University Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4546-8179","authenticated-orcid":false,"given":"Yigang","family":"Wang","sequence":"additional","affiliation":[{"name":"Xinyuan Institute of Medicine and Biotechnology School of Life Sciences and Medicine Zhejiang Sci\u2010Tech University Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,10,14]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2011.260"},{"key":"e_1_2_8_3_1","first-page":"1","article-title":"A new strategy for cancer therapy: Targeting gene\u2010virotherapy of cancer","volume":"8","author":"Liu X","year":"2001","journal-title":"Chinese Journal of Cancer. 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