{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T18:50:19Z","timestamp":1785351019329,"version":"3.55.0"},"reference-count":41,"publisher":"Wiley","issue":"23","license":[{"start":{"date-parts":[[2020,10,27]],"date-time":"2020-10-27T00:00:00Z","timestamp":1603756800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Oral squamous cell carcinoma (OSCC) is a prevalent cancer that develops in the head and neck area and has high annual mortality despite optimal treatment. microRNA\u2010218 (miR\u2010218) is a tumour inhibiting non\u2010coding RNA that has been reported to suppress the cell proliferation and invasion in various cancers. Thus, our study aims to determine the mechanism underlying the inhibitory role of miR\u2010218 in OSCC. We conducted a bioinformatics analysis to screen differentially expressed genes in OSCC and their potential upstream miRNAs. After collection of surgical OSCC tissues, we detected GREM1 expression by immunohistochemistry, RT\u2010qPCR and Western blot analysis, and miR\u2010218 expression by RT\u2010qPCR. The target relationship between miR\u2010218 and GREM1 was assessed by dual\u2010luciferase reporter gene assay. After loss\u2010 and gain\u2010of\u2010function experiments, OSCC cell proliferation, migration and invasion were determined by MTT assay, scratch test and Transwell assay, respectively. Expression of TGF\u2010\u03b21, Smad4, p21, E\u2010cadherin, Vimentin and Snail was measured by RT\u2010qPCR and Western blot analysis. Finally, effects of miR\u2010218 and GREM1 on tumour formation and liver metastasis were evaluated in xenograft tumour<jats:bold>\u2010<\/jats:bold>bearing nude mice. GREM1 was up\u2010regulated, and miR\u2010218 was down\u2010regulated in OSCC tissues, and GREM1 was confirmed to be the target gene of miR\u2010218. Furthermore, after up\u2010regulating miR\u2010218 or silencing GREM1, OSCC cell proliferation, migration and invasion were reduced. In addition, expression of TGF\u2010\u03b2 signalling pathway\u2010related genes was diminished by overexpressing miR\u2010218 or down\u2010regulating GREM1. Finally, up\u2010regulated miR\u2010218 or down\u2010regulated GREM1 reduced tumour growth and liver metastasis in vivo. Taken together, our findings suggest that the overexpression of miR\u2010218 may inhibit OSCC progression by inactivating the GREM1\u2010dependent TGF\u2010\u03b2 signalling pathway.<\/jats:p>","DOI":"10.1111\/jcmm.15972","type":"journal-article","created":{"date-parts":[[2020,10,27]],"date-time":"2020-10-27T15:07:21Z","timestamp":1603811241000},"page":"13824-13836","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Role of miR\u2010218\u2010GREM1 axis in epithelial\u2010mesenchymal transition of oral squamous cell carcinoma: An in vivo and vitro study based on microarray data"],"prefix":"10.1111","volume":"24","author":[{"given":"Yanpeng","family":"Wang","sequence":"first","affiliation":[{"name":"Department of E.N.T. Linyi People's Hospital  Linyi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifeng","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Stomatology Shandong Medical College  Linyi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7628-4148","authenticated-orcid":false,"given":"Long","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Stomatology Linyi People's Hospital  Linyi China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,10,27]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2010.03.009"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21332"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2982"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tripleo.2005.07.038"},{"key":"e_1_2_9_6_1","first-page":"985","article-title":"The utility of CD44, CD117 and CD133 in identification of cancer stem cells (CSC) in oral squamous cell carcinomas (OSCC)","volume":"52","author":"Margaritescu C","year":"2011","journal-title":"Rom J Morphol Embryol"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2009.01.004"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-015-4246-9"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.709"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.13755"},{"key":"e_1_2_9_11_1","first-page":"1419","article-title":"Clinicopathological and prognostic significance of epithelial gremlin1 expression in gastric cancer","volume":"38","author":"Honma R","year":"2018","journal-title":"Anticancer Res"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.21873\/cgp.20167"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.5808\/GI.2012.10.1.23"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.17.8.4801"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells8101130"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0925-4773(91)90027-4"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1042\/CS20120252"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/00054725-200403000-00016"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.3899\/jrheum.150523"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm995"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-10-0244"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.29148"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-020-2273-y"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.18095"},{"key":"e_1_2_9_25_1","first-page":"1035","article-title":"Semaphorin\u20107A contributes to growth, migration and invasion of oral tongue squamous cell carcinoma through TGF\u2010beta\u2010mediated EMT signaling pathway","volume":"22","author":"Liu TJGJ","year":"2018","journal-title":"Eur Rev Med Pharmacol Sci"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2018.02.104"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgu102"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.5732\/cjc.012.10219"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2012.1254"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.2013.1851"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-11-0368"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2015.3516"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2015.2216"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1000879"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00344.2016"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0174565"},{"key":"e_1_2_9_37_1","first-page":"13","article-title":"miR\u2010218 on the genomic loss region of chromosome 4p15.31 functions as a tumor suppressor in bladder cancer","volume":"39","author":"Tatarano SCT","year":"2011","journal-title":"Int J Oncol"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20140771"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2017.12.035"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2010.090747"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.3892\/or.2014.3657"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2010-11-8-r90"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.15972","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jcmm.15972","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.15972","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,12]],"date-time":"2024-01-12T12:04:27Z","timestamp":1705061067000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.15972"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10,27]]},"references-count":41,"journal-issue":{"issue":"23","published-print":{"date-parts":[[2020,12]]}},"alternative-id":["10.1111\/jcmm.15972"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.15972","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,10,27]]},"assertion":[{"value":"2020-06-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-09-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-10-27","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}