{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:07:16Z","timestamp":1789690036083,"version":"4.0.1"},"reference-count":40,"publisher":"Wiley","issue":"23","license":[{"start":{"date-parts":[[2020,10,22]],"date-time":"2020-10-22T00:00:00Z","timestamp":1603324800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Exosomes were found to exert a therapeutic effect in the treatment of osteonecrosis of the femoral head (ONFH), while miR\u2010135b was shown to play an important role in the development of ONFH. In this study, we investigated the effects of concomitant administration of exosomes and miR\u2010135b on the treatment of ONFH. A rat mode of ONFH was established. TEM, Western blotting and nanoparticle analysis were used to characterize the exosomes collected from human\u2010induced pluripotent stem cell\u2013derived mesenchymal stem cells (hiPS\u2010MSC\u2010Exos). Micro\u2010CT was used to observe the trabecular bone structure of the femoral head. Real\u2010time PCR, Western blot analysis, IHC assay, TUNEL assay, MTT assay and flow cytometry were performed to detect the effect of hiPS\u2010MSC\u2010Exos and miR\u2010135b on cell apoptosis and the expression of PDCD4\/caspase\u20103\/OCN. Moreover, computational analysis and luciferase assay were conducted to identify the regulatory relationship between PDCD4 mRNA and miR\u2010135b. The hiPS\u2010MSC\u2010Exos collected in this study displayed a spheroidal morphology with sizes ranging from 20 to 100\u00a0nm and a mean concentration of 1\u00a0\u00d7\u00a010<jats:sup>12<\/jats:sup> particles\/mL. During the treatment of ONFH, the administration of hiPS\u2010MSC\u2010Exos and miR\u2010135b alleviated the magnitude of bone loss. Furthermore, the treatment of MG\u201063 and U\u20102 cells with hiPS\u2010MSC\u2010Exos and miR\u2010135b could promote cell proliferation and inhibit cell apoptosis. Moreover, PDCD4 mRNA was identified as a virtual target gene of miR\u2010135b. HiPS\u2010MSC\u2010Exos exerted positive effects during the treatment of ONFH, and the administration of miR\u2010135b could reinforce the effect of hiPS\u2010MSC\u2010Exos by inhibiting the expression of PDCD4.<\/jats:p>","DOI":"10.1111\/jcmm.16006","type":"journal-article","created":{"date-parts":[[2020,10,22]],"date-time":"2020-10-22T14:19:29Z","timestamp":1603376369000},"page":"13973-13983","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Administration of mircoRNA\u2010135b\u2010reinforced exosomes derived from MSCs ameliorates glucocorticoid\u2010induced osteonecrosis of femoral head (ONFH) in rats"],"prefix":"10.1111","volume":"24","author":[{"given":"Xiang","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Orthopedics Wenzhou Hospital of Integrated Traditional Chinese and Western Medicine Zhejiang University of Traditional Chinese Medicine Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiong\u2010ming","family":"You","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Wenzhou Hospital of Integrated Traditional Chinese and Western Medicine Zhejiang University of Traditional Chinese Medicine Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao\u2010jun","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Wuhan Hospital of Traditional Chinese Medicine Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3809-3698","authenticated-orcid":false,"given":"Yang","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine of TCM Wenzhou Hospital of Integrated Traditional Chinese and Western Medicine Zhejiang University of Traditional Chinese Medicine Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,10,22]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201200373"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2014.1811"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12020-011-9580-0"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10067-014-2607-1"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10555-013-9441-9"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00281-010-0233-9"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1302\/2046-3758.56.BJR-2015-0014.R1"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1042\/BC20080191"},{"key":"e_1_2_9_10_1","first-page":"801","article-title":"MicroRNA206 contributes to the progression of steroidinduced avascular necrosis of the femoral head by inducing osteoblast apoptosis by suppressing programmed cell death 4","volume":"17","author":"Zhang Z","year":"2018","journal-title":"Mol Med Rep"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2014.11.004"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature03702"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0114627"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0079752"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.12138"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-015-0417-0"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.14809"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.16150"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19103119"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00264-018-3895-x"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.20759"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arth.2015.06.022"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.308.5730.1862"},{"key":"e_1_2_9_24_1","first-page":"177","article-title":"Reticulocyte membrane remodeling: contribution of the exosome pathway","volume":"17","author":"Blanc L","year":"2010","journal-title":"Curr Opin Hematol"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2011.12.037"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2012.00056"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbin.10869"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms2876"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2013.01.003"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-08-0951"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1208194"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19092791"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2014.03.006"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2013.02.046"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0102621"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.3858\/emm.2012.44.12.079"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10495-015-1201-6"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1177\/039463200501800311"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3302983"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.166157"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.16006","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jcmm.16006","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.16006","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,12]],"date-time":"2024-01-12T12:02:53Z","timestamp":1705060973000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.16006"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10,22]]},"references-count":40,"journal-issue":{"issue":"23","published-print":{"date-parts":[[2020,12]]}},"alternative-id":["10.1111\/jcmm.16006"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.16006","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,10,22]]},"assertion":[{"value":"2020-06-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-10-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-10-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}