{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T00:46:28Z","timestamp":1793321188587,"version":"4.5.0"},"reference-count":41,"publisher":"Wiley","issue":"23","license":[{"start":{"date-parts":[[2020,10,30]],"date-time":"2020-10-30T00:00:00Z","timestamp":1604016000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81970597"],"award-info":[{"award-number":["81970597"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81700662"],"award-info":[{"award-number":["81700662"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81630019"],"award-info":[{"award-number":["81630019"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81870519"],"award-info":[{"award-number":["81870519"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31430028"],"award-info":[{"award-number":["31430028"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Cumulative evidence suggests that abnormal differentiation of T lymphocytes influences the pathogenesis of chronic prostatitis\/chronic pelvic pain syndrome (CP\/CPPS). Thus, understanding the immune activation landscape of CP\/CPPS would be helpful for improving therapeutic strategies. Here, we utilized BD\u2122 AbSeq to digitally quantify both the protein and mRNA expression levels in single peripheral blood T cells from two CP\/CPPS patients and two healthy controls. We utilized an integrated strategy based on canonical correlation analysis of 10\u00a0000+ AbSeq profiles and identified fifteen unique T\u2010cell subpopulations. Notably, we found that the proportion of cluster 0 in the CP\/CPPS group (30.35%) was significantly increased compared with the proportion in the healthy control group (9.38%); cluster 0 was defined as effector T cells based on differentially expressed genes\/proteins. Flow cytometry assays confirmed that the proportions of effector T\u2010cell subpopulations, particularly central memory T cells, T helper (Th)1, Th17 and Th22 cells, in the peripheral blood mononuclear cell populations of patients with CP\/CPPS were significantly increased compared with those of healthy controls (\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0&lt;\u00a00.05), further confirming that aberration of effector T cells possibly leads to or intensifies CP\/CPPS. Our results provide novel insights into the underlying mechanisms of CP\/CPPS, which will be beneficial for its treatment.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.16021","type":"journal-article","created":{"date-parts":[[2020,10,30]],"date-time":"2020-10-30T04:29:21Z","timestamp":1604032161000},"page":"14099-14109","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Single\u2010cell multi\u2010omics analysis presents the landscape of peripheral blood T\u2010cell subsets in human chronic prostatitis\/chronic pelvic pain syndrome"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4935-4005","authenticated-orcid":false,"given":"Meng","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Urology The First Affiliated Hospital of Anhui Medical University Hefei China"},{"name":"Institute of Urology Anhui Medical University Hefei China"},{"name":"Anhui 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