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However, the molecular mechanisms whereby FOXD2\u2010AS1 is involved in recurrent pterygium remain unknown. Here, qRT\u2010PCR was performed to quantify FOXD2\u2010AS1 expression, while CCK\u20108, flow cytometer and neoplasm xenograft assays were used to investigate its function. Dual\u2010luciferase reporter, RIP and RNA pull\u2010down assays were conducted to address the relationship between FOXD2\u2010AS1, miR\u2010205\u20105p and VEGF\u2010A, while ChIP assays were used to detect H3K27 acetylation at the FOXD2\u2010AS1 promoter. FOXD2\u2010AS1 expression was up\u2010regulated in recurrent pterygium tissues. Moreover, a high FOXD2\u2010AS1 expression was associated with advanced stages, increased microvessel density and shorter recurrent\u2010free survival. In addition, ROC analysis showed that FOXD2\u2010AS1 is a valid predictor of recurrent pterygium. Furthermore, we show that FOXD2\u2010AS1 induced proliferation and inhibited apoptosis in a cell line derived from recurrent pterygia (HPF\u2010R) at least partially through the regulation of the miR\u2010205\u2010VEGF pathway. In addition, the up\u2010regulation of FOXD2\u2010AS1 was attributed to the H3K27 acetylation at the promoter region. In conclusion, FOXD2\u2010AS1 is activated via its H3K27 acetylation and regulates VEGF\u2010A expression by sponging miR\u2010205\u20105p in recurrent pterygium. Our results may provide a basis for the development of new therapeutic targets and biomarkers for recurrent pterygium.<\/jats:p>","DOI":"10.1111\/jcmm.16024","type":"journal-article","created":{"date-parts":[[2020,10,24]],"date-time":"2020-10-24T06:10:00Z","timestamp":1603519800000},"page":"14139-14151","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Activation of LncRNA FOXD2\u2010AS1 by H3K27 acetylation regulates VEGF\u2010A expression by sponging miR\u2010205\u20105p in recurrent pterygium"],"prefix":"10.1111","volume":"24","author":[{"given":"Yali","family":"Gao","sequence":"first","affiliation":[{"name":"Department of Ophthalmology Shenzhen People\u2019s Hospital (The Second Clinical Medical College, Jinan University) Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoling","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Shenzhen 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