{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T03:16:56Z","timestamp":1782789416947,"version":"3.54.5"},"reference-count":34,"publisher":"Wiley","issue":"24","license":[{"start":{"date-parts":[[2020,11,3]],"date-time":"2020-11-03T00:00:00Z","timestamp":1604361600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007219","name":"Natural Science Foundation of Shanghai","doi-asserted-by":"publisher","award":["14ZR1437100"],"award-info":[{"award-number":["14ZR1437100"]}],"id":[{"id":"10.13039\/100007219","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The incidence of syphilis caused by <jats:italic>Treponema pallidum<\/jats:italic> subsp <jats:italic>pallidum<\/jats:italic> (<jats:italic>T pallidum<\/jats:italic>) infection is accompanied by inflammatory injuries of vascular endothelial cells. Studies have revealed that <jats:italic>T pallidum<\/jats:italic> infection could induce inflammasome activation and pyroptosis in macrophages. MicroRNA\u2010223\u20103p (miR\u2010223\u20103p) was reported to be a negative regulator in inflammatory diseases. The present study aimed to explore whether miR\u2010223\u20103p regulates <jats:italic>T pallidum\u2010<\/jats:italic>induced inflammasome activation and pyroptosis in vascular endothelial cells, and determine the mechanisms which underlie this process. MiR\u2010223\u20103p levels in syphilis and control samples were determined. The biological function of miR\u2010223\u20103p in the NLRP3 inflammasome and pyroptosis was evaluated in <jats:italic>T pallidum\u2010<\/jats:italic>infected human umbilical vein endothelial cells (HUVECs). We observed a dramatic decrease in miR\u2010223\u20103p levels in syphilis patients (n\u00a0=\u00a020) when compared to healthy controls (n\u00a0=\u00a020). Moreover, miR\u2010223\u20103p showed a notable inhibitory effect on recombinant Tp17 (rTP17)\u2010induced caspase\u20101 activation, resulting in decrease in IL\u20101\u03b2 production and pyroptosis, which was accompanied by the release of lactate dehydrogenase (LDH) in HUVECs. Additionally, the dual\u2010luciferase assay confirmed that NLRP3 is a direct target of miR\u2010223\u20103p. Moreover, NLRP3 overexpression or knockdown largely blocked the effects of miR\u2010223\u20103p on <jats:italic>T pallidum<\/jats:italic>\u2010induced inflammasome activation and pyroptosis in HUVECs. Most importantly, a notable negative correlation was observed between miR\u2010223\u20103p and NLRP3, caspase\u20101, and IL\u20101\u03b2, respectively, in the serum of syphilis patients and healthy controls. Taken together, our results reveal that miR\u2010223\u20103p targets NLRP3 to suppress inflammasome activation and pyroptosis in <jats:italic>T pallidum<\/jats:italic>\u2010infected endothelial cells, implying that miR\u2010223\u20103p could be a potential target for syphilis patients.<\/jats:p>","DOI":"10.1111\/jcmm.16061","type":"journal-article","created":{"date-parts":[[2020,11,4]],"date-time":"2020-11-04T06:40:08Z","timestamp":1604472008000},"page":"14405-14414","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":69,"title":["MiR\u2010223\u20103p inhibits rTp17\u2010induced inflammasome activation and pyroptosis by targeting NLRP3"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2508-2391","authenticated-orcid":false,"given":"Fu\u2010quan","family":"Long","sequence":"first","affiliation":[{"name":"Department of STD Shanghai Skin Disease Hospital Tongji University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cai\u2010xia","family":"Kou","sequence":"additional","affiliation":[{"name":"Institute of Dermatology Chinese Academy of Medical Sciences and Peking Union Medical College  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke","family":"Li","sequence":"additional","affiliation":[{"name":"Department of STD Shanghai Skin Disease Hospital Tongji University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of STD Shanghai Skin Disease Hospital Tongji University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian\u2010qiu","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Dermatology Chinese Academy of Medical Sciences and Peking Union Medical College  Nanjing 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