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Considering cytomegalovirus (CMV) mainly invades through the respiratory tract, CMV\u2010specific pulmonary mucosal vaccine development that provides a long\u2010lasting protection against CMV challenge gains our attention. In this study, N\u2010terminal domain of GP96 (GP96\u2010NT) was used as a mucosal adjuvant to enhance the induction of pulmonary\u2010resident CD8 T cells elicited by MCMV glycoprotein B (gB) vaccine. Mice were intranasally co\u2010immunized with 50\u00a0\u03bcg pgB and equal amount of pGP96\u2010NT vaccine 4 times at 2\u2010week intervals, and then <jats:italic>i.n<\/jats:italic>. challenged with MCMV at 16\u00a0weeks after the last immunization. Compared with pgB immunization alone, co\u2010immunization with pgB\/pGP96\u2010NT enhanced a long\u2010lasting protection against MCMV pneumonitis by significantly improved pneumonitis pathology, enhanced bodyweight, reduced viral burdens and increased survival rate. Moreover, the increased CD8 T cells were observed in lung but not spleen from pgB\/pGP96\u2010NT co\u2010immunized mice. The increments of pulmonary CD8 T cells might be mainly due to non\u2010circulating pulmonary\u2010resident CD8 T\u2010cell subset expansion but not circulating CD8 T\u2010cell populations that home to inflammation site upon MCMV challenge. Finally, the deterioration of MCMV pneumonitis by depletion of pulmonary site\u2010specific CD8 T cells in mice that were pgB\/pGP96\u2010NT co\u2010immunization might be a clue to interpret the non\u2010circulating pulmonary\u2010resident CD8 T subset expansion. These data might uncover a promising long\u2010lasting prophylactic vaccine strategy against MCMV\u2010induced pneumonitis.<\/jats:p>","DOI":"10.1111\/jcmm.16065","type":"journal-article","created":{"date-parts":[[2020,11,6]],"date-time":"2020-11-06T08:58:02Z","timestamp":1604653082000},"page":"14426-14440","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["gB co\u2010immunization with GP96 enhances pulmonary\u2010resident CD8 T cells and exerts a long\u2010term defence against MCMV pneumonitis"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1148-7155","authenticated-orcid":false,"given":"Bingnan","family":"Guo","sequence":"first","affiliation":[{"name":"Jiangsu Institute of Health Emergency Xuzhou Medical University Xuzhou China"},{"name":"Emergency Center The Affiliated Hospital of Xuzhou Medical University Xuzhou 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