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Extracellular vesicles (EVs) carrying microRNA (miR) have been reported as therapeutic targets for liver diseases. In this study, we set out to explore whether adipose\u2010derived mesenchymal stem cells (ADMSCs)\u2010derived EVs containing miR\u2010150\u20105p affect the progression of HF. Carbon tetrachloride (CCl<jats:sub>4<\/jats:sub>) was firstly used to induce HF mouse models in C57BL\/6J mice, and activation of hepatic stellate cells (HSCs) was achieved using transforming growth factor \u03b2 (TGF\u2010\u03b2). EVs were then isolated from ADMSCs and co\u2010cultured with HSCs. The relationship between miR\u2010150\u20105p and CXCL1 was identified using dual luciferase gene reporter assay. Following loss\u2010 and gain\u2010function experimentation, HSC proliferation was examined by MTT assay, and levels of fibrosis\u2010, HSC activation\u2010 and apoptosis\u2010related genes were determined in vitro. Additionally, pathological scores, collagen volume fraction <jats:bold>(<\/jats:bold>CVF) as well as levels of inflammation\u2010 and hepatic injury\u2010associated genes were determined in in vivo. Down\u2010regulated miR\u2010150\u20105p and elevated CXCL1 expression levels were detected in HF tissues. ADMSCs\u2010derived EVs transferred miR\u2010150\u20105p to HSCs. CXCL1 was further verified as the downstream target gene of miR\u2010150\u20105p. Moreover, ADMSCs\u2010EVs containing miR\u2010150\u20105p markedly inhibited HSC proliferation and activation in vitro. Meanwhile, in vivo experiments also concurred with the aforementioned results as demonstrated by inhibited CVF, reduced inflammatory factor levels and hepatic injury\u2010associated indicators. Both experiments results were could be reversed by CXCL1 over\u2010expression. Collectively, our findings indicate that ADMSCs\u2010derived EVs containing miR\u2010150\u20105p attenuate HF by inhibiting the CXCL1 expression.<\/jats:p>","DOI":"10.1111\/jcmm.16119","type":"journal-article","created":{"date-parts":[[2020,12,20]],"date-time":"2020-12-20T16:09:40Z","timestamp":1608480580000},"page":"701-715","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":66,"title":["Extracellular vesicles\u2010derived miR\u2010150\u20105p secreted by adipose\u2010derived mesenchymal stem cells inhibits CXCL1 expression to attenuate hepatic fibrosis"],"prefix":"10.1111","volume":"25","author":[{"given":"Zhiyong","family":"Du","sequence":"first","affiliation":[{"name":"Department of Hepatobiliary and Pancreatic Surgery Shenzhen People\u2019s Hospital (The Second Clinical Medical College Jinan University Guangzhou China"},{"name":"The First Affiliated Hospital Southern University of Science and Technology) Shenzhen 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Hospital (The Second Clinical Medical College Jinan University Guangzhou China"},{"name":"The First Affiliated Hospital Southern University of Science and Technology) Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2058-3402","authenticated-orcid":false,"given":"Cuifeng","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Endocrinology Jingmen First People\u2019s Hospital Jingmen China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,12,20]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2016.08.023"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.30097"},{"issue":"11","key":"e_1_2_8_4_1","first-page":"1793","article-title":"Reference values of certain serum indicators of liver fibrosis","volume":"63","author":"Pencheva B","year":"2017","journal-title":"Clin 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