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It has been demonstrated that microRNA (miRNA) dysfunction plays an important role in the initiation and development of bone loss post\u2010SCI. Nevertheless, the effect of miR\u201019b\u20103p on bone loss after SCI is unknown and the accurate mechanism is left to be elucidated. The present work was conducted to explore the role of miR\u201019b\u20103p\/phosphatase and tensin homolog deleted on chromosome ten (PTEN) axis on osteogenesis after SCI and further investigates the underlying mechanisms. We found that miR\u201019b\u20103p level was increased in the femurs of SCI rats with decreased autophagy. The overexpression of miR\u201019b\u20103p in bone marrow mesenchymal stem cells (BMSCs) targeted down\u2010regulation of PTEN expression, facilitated protein kinase B (Akt) and mammalian target of rapamycin (mTOR) phosphorylation, and thereby suppressing BMSCs osteogenic differentiation via autophagy. Besides, the inhibiting effects of miR\u201019b\u20103p on osteogenic differentiation of BMSCs could be diminished by autophagy inducer rapamycin. Meanwhile, bone loss after SCI in rats was also reversed by antagomir\u201019b\u20103p treatment, suggesting miR\u201019b\u20103p was an essential target for osteogenic differentiation via regulating autophagy. These results indicated that miR\u201019b\u20103p was involved in bone loss after SCI by inhibiting osteogenesis via PTEN\/Akt\/mTOR signalling pathway.<\/jats:p>","DOI":"10.1111\/jcmm.16159","type":"journal-article","created":{"date-parts":[[2020,12,18]],"date-time":"2020-12-18T10:24:40Z","timestamp":1608287080000},"page":"990-1000","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["MiR\u201019b\u20103p accelerates bone loss after spinal cord injury by suppressing osteogenesis via regulating PTEN\/Akt\/mTOR signalling"],"prefix":"10.1111","volume":"25","author":[{"given":"Da","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Orthopedics Shengjing Hospital of China Medical University  Shenyang People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Ultrasound Shengjing 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