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To this effect, we deemed it necessary to identify potential B and T cell epitopes from the envelope S protein. This can be used as potential targets to develop anti\u2010SARS\u2010CoV\u20102 vaccine preparations. In this study, we used immunoinformatics to identify conservative B and T cell epitopes for S proteins of SARS\u2010CoV\u20102, which might play roles in the initiation of SARS\u2010CoV\u20102 infection. We identified the B cell and T cell peptide epitopes of S protein and their antigenicity, as well as the interaction between the peptide epitopes and human leucocyte antigen (HLA). Among the B cell epitopes, \u2018EILDITPCSFGGVS\u2019 has the highest score of antigenicity and great immunogenicity. In T cell epitopes, MHC\u2010I peptide \u2018KIADYNYKL\u2019 and MHC\u2010II peptide \u2018LEILDITPC\u2019 were identified as high antigens. Besides, docking analysis showed that the predicted peptide \u2018KIADYNYKL\u2019 was closely bound to the HLA\u2010A*0201. The results of molecular dynamics simulation through GROMACS software showed that \u2018HLA\u2010A*0201~peptide\u2019 complex was very stable. And the peptide we selected could induce the T cell response similar to that of SARS\u2010CoV\u20102 infection. Moreover, the predicted peptides were highly conserved in different isolates from different countries. The antigenic epitopes presumed in this study were effective new vaccine targets to prevent SARS\u2010CoV\u20102 infection.<\/jats:p>","DOI":"10.1111\/jcmm.16200","type":"journal-article","created":{"date-parts":[[2020,12,16]],"date-time":"2020-12-16T03:06:28Z","timestamp":1608087988000},"page":"1274-1289","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":45,"title":["T and B cell Epitope analysis of SARS\u2010CoV\u20102 S protein based on immunoinformatics and experimental research"],"prefix":"10.1111","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2963-1060","authenticated-orcid":false,"given":"Ziwei","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology Xiangya School of Medicine Central South University Changsha China"},{"name":"Department of Clinical Laboratory Third Xiangya Hospital Central South University Changsha China"},{"name":"Department of NHC Key Laboratory 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