{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T02:01:04Z","timestamp":1791684064289,"version":"4.3.4"},"reference-count":22,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2021,2,20]],"date-time":"2021-02-20T00:00:00Z","timestamp":1613779200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009702","name":"Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009702","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Motor neuron disease (MND) is a rare group of disorders characterized by degeneration of motor neurons (MNs). The most common form of MND, amyotrophic lateral sclerosis (ALS), is an incurable disease with a variable rate of progression. The search of robust biomarkers able to discriminate among different ALS forms is paramount to properly stratify patients, and to identify those who could most likely benefit from experimental therapies. Phosphorylated\u2010neurofilament heavy chain (p\u2010NfH) and neurofilament light chain (NfL) are neuron\u2010specific components of the cytoskeleton and may represent reliable markers of neuronal injury in neurological disorders. In this study, we described our cohort of ALS patients in order to investigate whether and how cerebrospinal fluid (CSF) p\u2010NfH and NfL levels may reflect progression rate, MN involvement and the extent of neurodegeneration. CSF p\u2010NfH and NfL were significantly increased in ALS compared with healthy and disease controls, including patients with other forms of MND, and were higher in patients with more aggressive disease course, reflecting progression rate. We also evaluated neurofilament diagnostic accuracy in our centre, identifying with high sensitivity and 100% specificity cut\u2010off values of 0.652\u00a0ng\/mL for CSF p\u2010NfH (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.0001) and of 1261\u00a0pg\/mL for NfL (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.0001) in discriminating ALS from healthy controls. CSF neurofilaments were significantly correlated with ALS progression rate. Overall, CSF neurofilaments appear to reflect the burden of neurodegeneration in MND and represent reliable diagnostic and prognostic biomarkers in ALS.<\/jats:p>","DOI":"10.1111\/jcmm.16240","type":"journal-article","created":{"date-parts":[[2021,2,21]],"date-time":"2021-02-21T03:48:21Z","timestamp":1613879301000},"page":"3765-3771","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Diagnostic and prognostic value of CSF neurofilaments in a cohort of patients with motor neuron disease: A cross\u2010sectional study"],"prefix":"10.1111","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8460-938X","authenticated-orcid":false,"given":"Delia","family":"Gagliardi","sequence":"first","affiliation":[{"name":"Dino Ferrari Centre Department of Pathophysiology and Transplantation (DEPT) University of Milan Milan 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