{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T11:47:26Z","timestamp":1776340046667,"version":"3.51.2"},"reference-count":46,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,12,28]],"date-time":"2020-12-28T00:00:00Z","timestamp":1609113600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Previous studies identified the involvement of phosphoinositide\u2010specific phospholipase C (PLC) \u03b31 in some events of chondrocytes. This study aims to investigate whether and how PLC\u03b31 modulates autophagy to execute its role in osteoarthritis (OA) progression. Rat normal or human OA chondrocytes were pretreated with IL\u20101\u03b2 for mimicking or sustaining OA pathological condition. Using Western blotting, immunoprecipitation, qPCR, immunofluorescence and Dimethylmethylene blue assays, and ELISA and transmission electron microscope techniques, we found that PLC\u03b31 inhibitor U73122 enhanced Collagen II, Aggrecan and GAG levels, accompanied with increased LC3B\u2010II\/I ratio and decreased P62 expression level, whereas autophagy inhibitor Chloroquine partially diminished its effect. Meanwhile, U73122 dissociated Beclin1 from Beclin1\u2010IP3R\u2010Bcl\u20102 complex and blocked mTOR\/ULK1 axis, in which the crosstalk between PLC\u03b31, AMPK, Erk and Akt were involved. Additionally, by haematoxylin and eosin, Safranin O\/Fast green, and immunohistochemistry staining, we observed that intra\u2010articular injection of Ad\u2010shPLC\u03b31\u20101\/2 significantly enhanced Collagen and Aggrecan levels, accompanied with increased LC3B and decreased P62 levels in a rat OA model induced by anterior cruciate ligament transection and medial meniscus resection. Consequently, PLC\u03b31 inhibition\u2010driven autophagy conferred cartilage protection against OA through promoting ECM synthesis in OA chondrocytes in vivo and in vitro, involving the crosstalk between PLC\u03b31, AMPK, Erk and Akt.<\/jats:p>","DOI":"10.1111\/jcmm.16245","type":"journal-article","created":{"date-parts":[[2020,12,29]],"date-time":"2020-12-29T08:04:46Z","timestamp":1609229086000},"page":"1531-1545","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["PLC\u03b31 inhibition\u2010driven autophagy of IL\u20101\u03b2\u2010treated chondrocyte confers cartilage protection against osteoarthritis, involving AMPK, Erk and Akt"],"prefix":"10.1111","volume":"25","author":[{"given":"Xiaolei","family":"Chen","sequence":"first","affiliation":[{"name":"Zhongshan Hospital Xiamen University Xiamen 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