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Glucocorticoid (GC) was used to treat SD rats and bone marrow\u2010derived mesenchymal stem cells (BMSCs) to construct SIONFH model in vitro and in vivo, respectively. The pathological injury of the femoral head in the SIONFH rats was detected via haematoxylin\u2010eosin (HE) staining and immunohistochemistry (IHC). The osteogenic differentiation, proliferation and apoptosis of BMSCs were detected. Western blot was used to detect Smad7, Bax, Bcl2 and Smad2\/3. The potential targets of circPVT1 and miR\u201021\u20105p were validated through luciferase reporter gene assay and RNA pull\u2010down assay, respectively. We found that CircPVT1 was decreased in the femoral head of SIONFH rats and GC\u2010treated BMSCs, while miR\u201021\u20105p was markedly up\u2010regulated. Overexpressed circPVT1 attenuated the apoptosis and cell viability inhibition of BMSCs induced by GC, while miR\u201021\u20105p up\u2010regulation had the opposite effects. What's more, the in vivo experiments confirmed that up\u2010regulating circPVT1 repressed osteonecrosis in SIONFH rats through repressing apoptosis. Mechanistically, circPVT1 functioned as a ceRNA of miR\u201021\u20105p, which targeted at the 3'untranslated region of Smad7. CircPVT1 enhancing Smad7 and mitigating GC activated TGF\u03b2\/Smad2\/3 pathway through inhibiting miR\u201021\u20105p. In conclusion, CircPVT1 exerts protective effects against SIONFH via modulating miR\u201021\u20105p\u2010mediated Smad7\/TGF\u03b2 pathway.<\/jats:p>","DOI":"10.1111\/jcmm.16294","type":"journal-article","created":{"date-parts":[[2021,3,18]],"date-time":"2021-03-18T07:21:41Z","timestamp":1616052101000},"page":"4608-4622","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["CircPVT1 up\u2010regulation attenuates steroid\u2010induced osteonecrosis of the femoral head through regulating miR\u201021\u20105p\u2010mediated Smad7\/TGF\u03b2 signalling pathway"],"prefix":"10.1111","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3424-8691","authenticated-orcid":false,"given":"Yangquan","family":"Hao","sequence":"first","affiliation":[{"name":"Department of Osteonecrosis and Joint Reconstruction Honghui Hospital Xi\u2019an Jiaotong University Health Science Center Xi\u2019an 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