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Currently, no effective medicines have been available for treating it. In our research, we examined the effects of polysaccharides extracted from <jats:italic>Balanophora polyandra Griff<\/jats:italic> (BPPs) on kidney fibrosis and epithelial to mesenchymal transition (EMT) in vivo and in vitro, aiming to explore the underlying mechanisms. By using the mice with unilateral urethral obstruction (UUO) as experimental subjects, we examined the medicinal values of BPPs on alleviating RIF. The effects of BPPs were evaluated by examining the histological staining and relative mRNA and protein expression levels of the related genes. The possible underlying mechanisms were further explored with human normal renal proximal tubular epithelia (HK\u20102 cells) as in vitro model. In UUO mice, BPP treatment could significantly alleviate interstitial fibrosis through reducing the components (Collagens I, III and IV) of extracellular matrix (ECM), and reducing the activation of fibroblasts producing these components, as revealed by inhibiting the hallmarks (fibronectin and \u03b1\u2010SMA) of fibroblast activation. Furthermore, BPP administration increased the expression levels of matrix metalloproteinases (MMPs) and declined those of tissue inhibitors of metalloproteinases (TIMPs). BPPs markedly ameliorated EMT in both the kidneys of UUO mice and TGF\u2010\u03b21 treated HK\u20102 cells. Moreover, BPP treatment decreased the expression levels of several transcriptional factors involved in regulating E\u2010cadherin expression, including snail, twist and ZEB1. Additionally, the Hedgehog pathway was found to be closely correlated with renal fibrosis and EMT. Altogether, our results clearly demonstrated that BPP treatment effectively inhibited the Hedgehog pathway both in renal tissues of UUO mice and TGF\u2010\u03b21\u2010treated HK\u20102 cells. Thus, BPPs ameliorated RIF and EMT in vivo and in vitro via suppressing Hedgehog signalling pathway.<\/jats:p>","DOI":"10.1111\/jcmm.16313","type":"journal-article","created":{"date-parts":[[2021,1,28]],"date-time":"2021-01-28T23:57:08Z","timestamp":1611878228000},"page":"2828-2840","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Polysaccharides extracted from <i>balanophora polyandra Griff<\/i> (BPP) ameliorate renal Fibrosis and EMT via inhibiting the Hedgehog pathway"],"prefix":"10.1111","volume":"25","author":[{"given":"Luoying","family":"Li","sequence":"first","affiliation":[{"name":"College of Medical Science China Three Gorges University Yichang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Zhou","sequence":"additional","affiliation":[{"name":"College of Traditional Chinese Medicine China Three Gorges University Yichang China"},{"name":"Yichang Hospital of Traditional Chinese Medicine Yichang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Psychiatry and Psychology Stomatological Hospital of Jingmen City Jingmen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yumin","family":"He","sequence":"additional","affiliation":[{"name":"College of Medical Science China Three Gorges University Yichang China"},{"name":"Third\u2010Grade Pharmacological Laboratory on Chinese Medicine Approved by State Administration of Traditional Chinese Medicine China Three Gorges University Yichang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Xiao","sequence":"additional","affiliation":[{"name":"College of Medical Science China Three Gorges University Yichang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fan","family":"Peng","sequence":"additional","affiliation":[{"name":"College of Medical Science China Three Gorges University Yichang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0348-3146","authenticated-orcid":false,"given":"Chengfu","family":"Yuan","sequence":"additional","affiliation":[{"name":"College of Medical Science China Three Gorges University Yichang China"},{"name":"Third\u2010Grade Pharmacological Laboratory on Chinese Medicine Approved by State Administration of Traditional Chinese Medicine China Three Gorges University Yichang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,1,28]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(16)32064-5"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/kisup.2014.21"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2016.12.011"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.ASN.0000106015.29070.E7"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2010.05.002"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1254\/jphs.14173FP"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2015.07.040"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ki.5000054"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.11.007"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2009.12"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2009.01.033"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2011060614"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2014.11.004"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00232.2015"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2011.12.039"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2015.10.007"},{"key":"e_1_2_9_18_1","unstructured":"JingF YouP ZhaoWW FanHJ SunZL YuanL.Chemical Constituents of Balanophora involucrata. 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