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Exosomal miRNAs have emerged as new components in intercellular communication. However, their roles in the differentiation of SSC remain unclear. Here, we observed miR\u2010486\u20105p enriched in Sertoli cell and Sertoli cell\u2010derived exosomes. The exosomes mediate the transfer of miR\u2010486\u20105p from Sertoli cells to SSCs. Exosomes release miR\u2010486\u20105p, thus up\u2010regulate expression of\n                    <jats:italic>Stra8<\/jats:italic>\n                    (stimulated by retinoic acid 8) and promote differentiation of SSC. And PTEN was identified as a target of miR\u2010486\u20105p. Overexpression of miR\u2010486\u20105p in SSCs down\u2010regulates PTEN expression, which up\u2010regulates the expression of STRA8 and SYCP3, promotes SSCs differentiation. In addition, blocking the exosome\u2010mediated transfer of miR\u2010486\u20105p inhibits differentiation of SSC. Our findings demonstrate that miR\u2010486\u20105p acts as a communication molecule between Sertoli cells and SSCs in modulating differentiation of SSCs. This provides a new insight on molecular mechanisms that regulates SSC differentiation and a basis for the diagnosis, treatment, and prevention of male infertility.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.16347","type":"journal-article","created":{"date-parts":[[2021,2,20]],"date-time":"2021-02-20T22:13:58Z","timestamp":1613859238000},"page":"3950-3962","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":56,"title":["Sertoli cell\u2010derived exosomal MicroRNA\u2010486\u20105p regulates differentiation of spermatogonial stem cell through PTEN in mice"],"prefix":"10.1111","volume":"25","author":[{"given":"Quan","family":"Li","sequence":"first","affiliation":[{"name":"Department of Cell Biology &amp; Guangdong Provincial Key Laboratory of Bioengineering Medicine Jinan University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanhao","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Jinan University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinlian","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Cell Biology &amp; Guangdong Provincial Key Laboratory of Bioengineering Medicine Jinan University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaxin","family":"Mei","sequence":"additional","affiliation":[{"name":"Department of Cell Biology &amp; Guangdong Provincial Key Laboratory of Bioengineering Medicine Jinan University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Cell Biology &amp; Guangdong Provincial Key Laboratory of Bioengineering Medicine Jinan University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Institute of Biological Products and Materia Medica Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiao","family":"Luo","sequence":"additional","affiliation":[{"name":"Institute for Translational Medicine Shenzhen Second People\u2019s Hospital \/ The First Affiliated Hospital of Shenzhen University Health Science Center Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Cell Biology &amp; 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