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Several microRNAs regulate the activation and proliferation of HSCs, thereby playing a critical role in HF progression. Previous studies have reported that miR\u2010188\u20105p is dysregulated during the process of HF. However, the role of miR\u2010188\u20105p in HF remains unclear. This study investigated the potential role of miR\u2010188\u20105p in HSCs and HF. Firstly, we validated the miR\u2010188\u20105p expression in primary cells isolated from liver of carbon tetrachloride (CCl<jats:sub>4<\/jats:sub>)\u2010induced mice, TGF\u2010\u03b21\u2010induced LX\u20102 cells, livers from 6\u2010month high\u2010fat diet (HFD)\u2010induced rat and 4\u2010month HFD\u2010induced mice NASH models, and human non\u2010alcoholic fatty liver disease (NAFLD) patients. Furthermore, we used miR\u2010188\u20105p inhibitors to investigate the therapeutic effects of miR\u2010188\u20105p inhibition in the HFD\u00a0+\u00a0CCl<jats:sub>4<\/jats:sub> induced in vivo model and the potential role of miR\u2010188\u20105p in the activation and proliferation of HSCs. This present study reported that miR\u2010188\u20105p expression is significantly increased in the human NAFLD, HSCs isolated from liver of CCl<jats:sub>4<\/jats:sub> induced mice, and in vitro and in vivo models of HF. Mimicking the miR\u2010188\u20105p resulted in the up\u2010regulation of HSC activation and proliferation by directly targeting the phosphatase and tensin homolog (PTEN). Moreover, inhibition of miR\u2010188\u20105p reduced the activation and proliferation markers of HSCs through PTEN\/AKT pathway. Additionally, in vivo inhibition of miR\u2010188\u20105p suppressed the HF parameters, pro\u2010fibrotic and pro\u2010inflammatory genes, and fibrosis. Collectively, our results uncover the pro\u2010fibrotic role of miR\u2010188\u20105p. Furthermore, we demonstrated that miR\u2010188\u20105p inhibition decreases the severity of HF by reducing the activation and proliferation of HSCs through PTEN\/AKT pathway.<\/jats:p>","DOI":"10.1111\/jcmm.16376","type":"journal-article","created":{"date-parts":[[2021,3,10]],"date-time":"2021-03-10T14:47:22Z","timestamp":1615387642000},"page":"4073-4087","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["Inhibition of miR\u2010188\u20105p alleviates hepatic fibrosis by significantly reducing the activation and proliferation of HSCs through PTEN\/PI3K\/AKT pathway"],"prefix":"10.1111","volume":"25","author":[{"given":"Farooq","family":"Riaz","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology School of Basic Medical Sciences Xi\u2019an Jiaotong University Health Science Center  Xi\u2019an China"},{"name":"Key Laboratory of Environment and Genes Related to Diseases Ministry of 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