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Single\u2010cell RNA sequencing (scRNA\u2010seq) emerges to be a powerful tool to evaluate the full heterogeneity of kidney diseases. To reveal cellular gene expression profiles of glomerulonephritis, we performed scRNA\u2010seq of 2 human kidney transplantation donor samples, 4 human glomerulonephritis samples, 1 human malignant hypertension (MH) sample and 1 human chronic interstitial nephritis (CIN) sample, all tissues were taken from the biopsy. After filtering the cells with\u00a0&lt;\u00a0200 genes and\u00a0&gt;\u00a010% mitochondria (MT) genes, the resulting 14\u00a0932 cells can be divided into 20 cell clusters, consistently with the previous report, in disease samples dramatic immune cells infiltration was found, among which a proximal tubule (PT) subset characterized by wnt\u2010\u03b2 catenin activation and a natural killer T (NKT) subset high expressing LTB were found. Furthermore, in the cluster of the podocyte, three glomerulonephritis related genes named FXYD5, CD74 and B2M were found. Compared with the mesangial of donor, the gene CLIC1 and RPS26 were up\u2010regulated in mesangial of IgA nephropathy(IgAN), whereas the gene JUNB was up\u2010regulated in podocyte of IgAN in comparison with that of donor. Meanwhile, some membranous nephropathy (MN) high expressed genes such as HLA\u2010DRB5, HLA\u2010DQA2, IFNG, CCL2 and NR4A2, which involve in highest enrichment pathway, display the cellular\u2010specific expression style, whereas monocyte marker of lupus nephritis (LN) named TNFSF13B was also found and interferon alpha\/beta signalling pathway was enriched in B and NKT of LN comparing with donor. By scRNA\u2010seq, we first defined the podocyte markers of glomerulonephritis and specific markers in IgA, MN and LN were found at cellular level. Furthermore, the critical role of interferon alpha\/beta signalling pathway was enriched in B and NKT of LN was declared.<\/jats:p>","DOI":"10.1111\/jcmm.16407","type":"journal-article","created":{"date-parts":[[2021,3,23]],"date-time":"2021-03-23T04:22:15Z","timestamp":1616473335000},"page":"4684-4695","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["A single\u2010cell survey of the human glomerulonephritis"],"prefix":"10.1111","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0888-7876","authenticated-orcid":false,"given":"Zhejun","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Nephrology Molecular Cell Laboratory for Kidney Disease Ren Ji Hospital School of Medicine Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shanghai Institute of 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