{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T20:41:34Z","timestamp":1790628094034,"version":"4.1.0"},"reference-count":41,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2021,5,11]],"date-time":"2021-05-11T00:00:00Z","timestamp":1620691200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The objective of the study is to evaluate the protective effects of human mesenchymal stem cells (hMSCs) modified with miR\u2010138\u20105p inhibitor against the allergic rhinitis and asthma syndrome (ARAS). MiR\u2010138\u20105p or negative control was transfected into hMSCs, and fluorescence\u2010activated cell sorting was used to evaluate hMSC surface markers. Quantitative real\u2010time PCR (qRT\u2010PCR) was used to evaluate miR\u2010138\u20105p, <jats:italic>SIRT1<\/jats:italic>, caspase\u20103, IL\u20106, IL\u20101\u03b2 and TNF\u2010\u03b1 levels after TNF\u2010\u03b1 and IL\u20106 stimulations. hMSCs with or without miR\u2010138\u20105p inhibition was intranasally administered into ARAS mice (n\u00a0=\u00a010 each group), followed by monitoring sneezing and nasal rubbing events to evaluate the allergic symptoms. Histamine, ovalbumin\u2010specific IgE, IgG2a, IgG1 and LTC4 release were monitored in the serum and nasal lavage fluid using enzyme\u2010linked immunosorbent assay. Expression of <jats:italic>SIRT1<\/jats:italic> and HMGB1\/TLR4 pathway in nasal mucosa was assessed. After miR\u2010138\u20105p inhibitor transfection, the hMSC lineage was preserved. Binding between <jats:italic>SIRT1<\/jats:italic> and miR\u2010138\u20104p was observed, and miR\u2010138\u20105p inhibition led to upregulation of <jats:italic>SIRT1<\/jats:italic>. Inhibition of miR\u2010138\u20105p led to attenuated inflammatory responses of hMSCs upon TNF\u2010\u03b1 and IL\u20106 stimulation, and allergic symptoms in mice, as well as histamine and ovalbumin\u2010specific IgG release. hMSCs with miR\u2010138\u20105p inhibition showed characteristics of activated <jats:italic>SIRT1<\/jats:italic> and inhibited HMGB1\/TLR4 pathway. Inhibition of miR\u2010138\u20105p in hMSCs enhanced its effects in attenuating inflammatory responses and allergic reaction in the ARAS model, which is presumably regulated by <jats:italic>SIRT1<\/jats:italic> and the HMGB1\/TLR4 pathway.<\/jats:p>","DOI":"10.1111\/jcmm.16473","type":"journal-article","created":{"date-parts":[[2021,5,11]],"date-time":"2021-05-11T17:11:43Z","timestamp":1620753103000},"page":"5038-5049","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Protective effect of miR\u2010138\u20105p inhibition modified human mesenchymal stem cell on ovalbumin\u2010induced allergic rhinitis and asthma syndrome"],"prefix":"10.1111","volume":"25","author":[{"given":"Huaping","family":"Tang","sequence":"first","affiliation":[{"name":"Department of Respiratory Medicine Qingdao Municipal Hospital Qingdao 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