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In this study, we established three mice groups (control group, ADR\u2010R group and ADR\u2010R\u00a0+\u00a0shLINC00470 group). We detected PTEN mRNA expression in the presence of LINC00470 in the mice models, as well as in the KCL22 and K562 cells. LINC00470 was significantly enriched for PTEN mRNA to exhibit a negative regulatory relationship between LINC00470 and PTEN mRNA. However, the alteration of LINC00470 had no effect on the luciferase activity of PTEN promoter, while the half\u2010life of PTEN mRNA was affected. It was further validated that LINC00470 down\u2010regulated PTEN expression by positively regulating the m6A modification of PTEN mRNA via RNA methyltransferase METTL3. Moreover, the relative expression of LC3II, Beclin\u20101, ATG7 and ATG5 was all decreased in cells treated with LINC00470, and down\u2010regulated PTEN expression was observed in chemo\u2010resistant cells, while the expression of PTEN was rescued by the transfection of shMETTL3 into chemo\u2010resistant cells. Moreover, the knockdown of METTL3 also restored the normal level of PTEN m<jats:sup>6<\/jats:sup>A modification and LINC00470 expression in chemo\u2010resistant cells. In conclusion, our results demonstrated the molecular mechanism underlying the effect of LINC00470 on CML by reducing the PTEN stability via RNA methyltransferase METTL3, thus leading to the inhibition of cell autophagy while promoting chemoresistance in CML.<\/jats:p>","DOI":"10.1111\/jcmm.16478","type":"journal-article","created":{"date-parts":[[2021,3,22]],"date-time":"2021-03-22T02:24:20Z","timestamp":1616379860000},"page":"4248-4259","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Dysregulation of LINC00470 and METTL3 promotes chemoresistance and suppresses autophagy of chronic myelocytic leukaemia cells"],"prefix":"10.1111","volume":"25","author":[{"given":"Xun","family":"Lai","sequence":"first","affiliation":[{"name":"Department of Hematology The Third Affiliated Hospital of Kunming Medical University (Tumor Hospital of Yunnan Province) Kunming 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